Genotypic and Antibiotic Resistance Patterns of blaTEM, blaCTX and blaSHV Producing Klebsiella pneumoniae Isolates in Abdul Moeloek Hospital, Lampung, Indonesia
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 11
Abstract
The incidence of extended spectrum beta-lactamases (ESBL)-producing strains has been steadly increasing during the past few years, and remains an important cause of treatment failures with cephalosporin. This study intended to investigate the antibiotic resistance patterns and gene types of ESBL-producing Klebsiella pneumoniae isolates in Dr. H. Abdul Moeloek Hospital, Lampung, Indonesia.Klebsiella pneumoniae isolates (N=90) collected from various clinical samples such as pus, sputum, blood, wound swab, urine and body fluids were subjected to usual antibiotics using disc diffusion methods according to NCCLS criteria for resistance analysis.To determine the minimum inhibitory concentration (MIC) and identify the ESBL-producing strains, the isolates were confirmed with automated Vitek-2 system. Betalactamase production was assessed using double disk sinergy test (DDST) methods. While blaTEM, blaSHV, and blaCTX genotypes were determined by polymerase chain reaction (PCR) techniques. The ESBL producing Klebsiella pneumoniae strain was detected in 42 (46.7%) isolates of K. pneumoniae. High resistance of the isolates to the antibiotics was seen consecutively in ampicillin (100%), amoxycillin (78.8%), ceftazidime (57.8%), ceftriaxone (56.7%), cefepime (56.7%), aztreonam 56.7%, cefotaxime (55.6%) and cefazolin (51.5%). Susceptibility of the isolates to amikacin, ertapenem and meropenem is 96.6%, 94.4% and 94.4% respectively. The ESBL genotypes detected in the isolates consecutively are blaSHV (86.7% ), blaTEM (60.0%) and blaCTX (45.6%). It is now obvious from this research findings that ESBL producing K. pneumoniae has increased so that therapeutic strategies need to be carefully formulated to control infections by reliable laboratory methods.
Authors and Affiliations
Hidayat Rahman, Ellyza Nasrul, Djong Hon Tjong, Netti Suharti
Keywords
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- EP ID EP238764
- DOI 10.9790/0853-1611060611
- Views 108
- Downloads 0
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