Genotyping of Human papilloma virus DNA isolated from cervical tissue biopsies of Iraqi women with different cervical abnormalities
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
Human papillomaviruses(HPV) are the etiological agents of cervical and other ano-genital malignancies. More than 200 genotypes of HPV have been identified, of these, genotypes 16,18,31,33,45,51,52,53,58 have been classified as “high-risk” (HR-HPV) because they are associated with the malignant progression of cervical tumors and with other genital and head-neck malignancies. This study aimed to evaluate the association between the infection with HR-HPVs and histo-pathological presentations. Material and method: This cross-section and prospective study was conducted during March 2016 to March 2017.The study included 150 fresh cervical tissue biopsy samples collected from hysterectomy done for women with different cervical abnormalities. All samples were diagnosed histo-pathologically and classified into two groups; group of patients whom have cervical abnormalities and control group which includes samples with unremarkable changes taken from women by hysterectomy done for reasons other than cervical abnormalities. DNA was extracted from cervical tissue biopsy samples and the integrity was estimated by amplification of glyceraldehyde phosphate dehydrogenase (GAPDH) gene. Detection of HPV was done by PCR amplification using HPV-specific primer sets of MY09/MY11 gene. DNA samples show positivity for the above two reactions were further amplified for genotyping using specific primer sets for the HR-HPV genotypes (16, 18, 31, 35, 45, 51, 52, and 66). Results: Only 90/150 DNA samples were included in the results of this study. The most important histopathological findings were 6/90(6.7%) patients with squamous cell carcinoma(SCC), as a total, HPV-16 detected in 3/6(50%) patients, HPV-45 detected in 3/6(50%) patients and HPV-18 detected in 2/6 (33.3%) patients. As co-infection with HR-HPV genotypes, 2/6(33.33 %) patients have co-infection with genotypes (HPV16, 18, 45) and only 1(16.66%) patient has a co- infection with two HPV genotypes (HPV 16, 45). Risk estimation for SCC with respect to HPV-16,18,45 were significant (P<0.001).Conclusion: However, only a minority of pre-cancerous lesions progress to cervical cancer,HPV genotyping is an important risk factor for differentiation progression. Testing of HPV DNA should introduced into clinical practice in Iraq with the aim of monitor women whom are at risk of cervical cancer.
Authors and Affiliations
Hiba Sabah Jasim, Maysaa Abdul Razzaq Dhahi2, Thuraya Hussam Al-Deen Abdullah3
Keywords
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- EP ID EP391174
- DOI 10.9790/3008-1205046975
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