Gerbera jamessonii H. Bolus micropropagation
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 3
Abstract
Gerbera is one of the horticultural crops of great demand on the Cuban local market, but its exploitation is limited because with the traditional multiplication methods established, it is not possible to comply with the demands of the seeds. This work was carried out at the biofactory of Las Flores de Servicios Comunales, with the objective of establishing a methodology for the in vitro commercial propagation of Gerbera. Culture media and explant management in the different micropropagation phases were studied. The results obtained showed that with sodium hypochlorite at 0.5% during 10 minutes, a high disinfection of the shoot tips was achieved. With the combination of 6-bencilaminepurine (1.0 mg-l-1) and giberrelic acid (0.1 mg.l-1) in the culture medium, it was possible to increase the number of shoots per explant during establishment. In the multiplication phase, the best results were obtained with a combination of 6-BAP (2.0 mg.l-1) with AIA (0.65 and 1.3 mg.l-1) subculturing the explants greater than 1.0 cm individually in semisolid culture medium. A significant influence on the number of roots and growth of the explants was achieved adding indolacetic acid to the rooting medium and using for the subculture, explants greater than 3.0 cm. The quality of the explants influenced significantly in the acclimatization phase, demonstrating that the plants rooted in vitro should be 3.0 cm in height.
Authors and Affiliations
Pablo Machado Armas, Daniel Agramante, Nelly Almanza Sánches, Drialys Díaz Sánchez, Leinys Gómez Fleites
Keywords
Related Articles
- EP ID EP697813
- DOI -
- Views 67
- Downloads 0
In vitro multiplication of Zantedeschia spp. variety Treasure in Temporary Immersion Systems
Callas (Zantedeschia spp.) are plants from Araceae family of a great interest as ornamental plants in pots and as cut flowers. Although they have been propagated by in vitro culture, there are few scientific studies on...
Oxidative burst in plant-pathogen interaction
The production of reactive oxygen species (ROS) via consumption of oxygen in a so-called oxidative burst is a hallmark of successful recognition of infection and activation of plant defenses. ROS are not only toxic by-...
In vitro establishment of Eugenia squarrosa: an endemic species of Santa Clara (Cuba) in n extinction danger
The tissue culture techniques can be supplemented with others that are carried out in the cultivation in situ and being applied in combined way to give solution to the extinction of different species. Eugenia squarrosa...
Use of Temporary Immersion Systems for the large-scale production of in vitro tubers of Solanum tuberosum L. var. Atlantic and study of its behavior in the field
A special temporary immersion system was designed in order to scale up the potato in vitro tubers production as an alternative method for potato seed production. An average of 2.6 in vitro tubers with an average fresh we...
Genetic transformation by Agrobacterium tumefaciens of embryogenic cell suspensions of banana cultivar Grandenaine (AAA)
The black Sigatoka (Mycosphaerella fijiensis Morelet) has become in the last years, the most destructive disease that affects the production of banana and plantains world-wide. The present work was made with the objecti...