Germination of somatic embryos of Swietenia macrophylla in semi-solid culture media
Journal Title: Biotecnología Vegetal - Year 2005, Vol 5, Issue 1
Abstract
With the objective to establish a suitable culture medium and to determine the optimum culture time for the germination of somatic embryos of Swietenia macrophylla King, it was carried out these work. Somatic embryos at the cotyledonal stage were placed in a culture medium formed by the half of the MS salts for their germination . three concentrations of 6-BAP (0.2, 0.4 and 0.6 mg.l -1 ), two concentrations of AG (1.0 and 2.0 mg.l -1 ) and a culture medium control without 3 growth regulators were studied. In the culture medium where the best results were obtained, the effect of the culture time on the germination of somatic embryos (30, 60 and 90 days of culture) was studied. The major number of somatic embryos with total germination was obtained in the culture medium without growth regulators, with significant differences to the rest of the treatments. The highest value of somatic embryos with secondary embryogenesis was presented when 0.6 mg.l -1 of 6-BAP were added to the culture medium. The increase of the culture time to 60 and 90 days increased significantly the total germination of the somatic embryos. These results allowed to define a culture medium for the germination of somatic embryos of Swietenia macrophylla King composed by the half of the MS salts without growth regulators with a culture time of 60 days.
Authors and Affiliations
Raúl Collad*, Raúl Barbón, Daniel Agramonte, Felipe Jiménez, Martha Pérez, Odalys Gutiérrez
Keywords
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