Growth Kinetics and Metronidazole Sensitivity of Blastocystis Sp. Isolated from Colorectal Carcinoma (CRC) and Non-CRC Patients
Journal Title: The Egyptian Journal of Hospital Medicine - Year 2018, Vol 72, Issue 1
Abstract
<span>Blastocystis </span><span>sp. is the most common protist detected in human fecal samples in numerous studies globally. Extreme debate regarding </span><span>Blastocystis </span><span>sp. pathogenicity exists. Some studies have speculated a possible correlation between </span><span>Blastocystis </span><span>and CRC. No previous study has investigated the presence of non-genotypic differences in </span><span>Blastocystis </span><span>sp. isolated from CRC and non-CRC patients. The present work aimed to investigate the growth kinetics (G.K) and metronidazole (MTZ) sensitivity in </span><span>Blastocystis </span><span>isolates from CRC and apparent non-CRC whether symptomatic and asymptomatic </span><span>Blastocystis </span><span>carriers. Seven isolates from CRC patients and 6 from symptomatic and 6 from asymptomatic non-CRC carriers were cultured in Locke's Egg (LE) medium supplemented with bovine serum and antibiotic mixture and incubated at 37</span><span>◦ </span><span>C. Mean viable organisms counts of each isolate were counted every 24 hours in medium to follow their GK and after exposure to different MTZ concentrations to perform drug sensitivity assay. </span><span>In vitro </span><span>growth kinetics of CRC and Non-CRC symptomatic isolates were nearly similar with higher peaks attained at 72 hours incubation than by the slower growing non-CRC-asymptomatic isolates. MTZ-sensitivity of CRC isolates was nearly similar to that of non-CRC asymptomatic isolates, both were significantly more sensitive than the symptomatic isolates especially at high drug concentration of 200 μg/ml of the medium. </span>
Authors and Affiliations
Eman Helal
Keywords
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