Haemolymph composition of Ancylostomia stercorea Zeller (Lepidoptera:Pyralidae) larvae with particular reference to proteins and amino acids
Journal Title: Journal of Research in Biology - Year 2012, Vol 2, Issue 3
Abstract
Haemolymph of 3rd, 4th and 5th larval instars of A. stercorea was extracted and used in the identification of amino acids by electrophoresis, total protein content by Lowry’s method and proteins by SDS-PAGE. Potassium, sodium and glucose levels were also determined. Seventeen amino acids were identified in all three larval instars. Thirteen protein bands were present in the haemolymph of 4th and 5th larval instars, five of which had molecular weights of >45.4 kDa while ten protein bands were present in the haemolymph of 3rd larval instar. Sodium:Potassium ratios in 3rd, 4th and 5th larval instars were 0.05, 0.14 and 0.13 respectively. Glucose concentrations in 3rd, 4th and 5th larval instars were 0.16, 0.33 and 0.57 mg/ml respectively. The results provided critical information that can be used to create artificial diets for in-vitro rearing of A. stercorea parasitoids
Authors and Affiliations
Labban O, Jugmohan H, Khan A, Matthew J, Wisdom S
Keywords
Related Articles
- EP ID EP564637
- DOI -
- Views 109
- Downloads 0
Effect of feeding frequency on growth performances and survival of Rutilus rutilus caspicus
Feed management plays a major role in the economical and environmental status of fish farms. Optimum feeding frequency seems to be dependent on fish size and higher frequency of feeding was found to be advantageous for h...
Batasio flavus, a new catfish species (Siluriformes: Bagridae) from Kerala, India
Batasio flavus, a new species of the family Bagridae, is described from Manimala River of Kerala, India. It is distinguished from its relative species by the following combination of characters: body slender, dorsal prof...
Bioaccumulation of lead by Bacillus species isolated from pig waste
Heavy metals, such as lead, copper, cadmium, chromium and mercury are important environmental pollutants, particularly in areas with high anthropogenic pressure. Their presence in the atmosphere, soil and water, even in...
In vitro assessment of water current on growth and biometric relationship among molluscs
Water current plays vital role in the development of an aquatic ecosystem. It performs various activities in the aquatic media, which in turns replenish the nutrients and alter biotic conditions of the water bodies. In o...
Plasma protein markers for estimating the population genetic structure of Amazon turtle (Podocnemis expansa Schweigger, 1812)
This study aims to test the potential of allelic frequency distributions of plasma proteins analysed by starch gel electrophoresis for identifying Amazon turtle (Podocnemis expansa Schweigger, 1812) stocks. Three protein...