HBsAg non-reactive but HBV DNA reactive blood donors in Wuhu area: infection status and tracing results
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 1
Abstract
Objective To detect and analyze the infection status of HBsAg non-reactive /HBV DNA reactive blood donors by individual donor-NAT (ID-NAT) and chemiluminescence technology, and to explore the feasibility and potential risks of reentry. Methods The blood screening results of blood donors in Wuhu from January 2018 to October 2021 were queried by blood station information management software. The blood donation information of all HBsAg non-reactive /HBV DNA reactive blood donors was collected and then recalled by telephone. After informed consent, samples were taken for HBV DNA nucleic acid single test, enzyme-linked immunoassay for HBsAg, chemiluminescence assay for HBV seromarkers(including HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc), and alanine aminotransferase (ALT) test. All the results were statistically analyzed. Results From January 2018 to October 2021, there were 142 051 donations, and the positive rate of sole HBV DNA was 0.06% (91/142 051), and 33 people (37 person-times) were successfully followed up. The yield rates of HBsAg, anti-HBs and anti-HBc were 6.06% (2/33), 39.39% (13/33) and 96.97% (32/33), respectively; None HBeAg was yielded. After two times of ID-NAT, 8 patients remained non-reactive to both systems, with a negative conversion rate of 24.24% (8/33). Meanwhile, 25 patients were at least once reactive to ID-NAT, and 23 of them were occult HBV infection with serologically reactivity. There were 2(6.25%) patients with HBsAg positive conversion and HBV DNA persistent reactivity, which were window period infection. One person was confirmed as false reactivity (no HBV infection) as he remained unreactive to both repeated ID-NAT and serological tests. Conclusion Chemiluminescence assay is more sensitive than ELISA in detecting HBV serum markers, which is beneficial to early detection of HBV samples in window period. The yielding rate of anti-HBc among HBsAg non-reactive/HBV DNA reactive blood donors detected by blood screening in this region is very high, and most of them are occulting infection, so the ID-NAT should be no less than 2 times in the reentry strategy.
Authors and Affiliations
Jie WU, Jie PAN, Guoqing YU, Ying ZHANG, Hui HUANG, Yi MA, Song YUAN, Xu YANG
Keywords
Related Articles
- EP ID EP738467
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.01.016
- Views 33
- Downloads 0
Molecular mechanism of ABO bood group variation
Objective To study the relationship between ABO subtype, para-Bombay blood group and genotype, so as to explore the possible molecular mechanism of these two blood groups, and provide accurate genetic detection targets a...
Nursing care of apheresis platelet donor who developed red blood cell spillage: a case report and literature review
Objective To explore the causes and nursing strategies of red blood cell spillage in apheresis platelet donors, so as to avoid adverse reactions to blood donation and platelet discarding and improve blood donation servic...
Construction of a consistency evaluation system for a nucleic acid testing kit: a preliminary study
Objective To evaluate the performance of a blood nucleic acid testing kit, construct a consistency assessment system, and analyze the influencing factors of the test results. Methods A total of 18 644 samples of voluntar...
Research progress of IgM-enriched human intravenous immunoglobulin products
Comparing with conventional human intravenous immunoglobulin products (hIVIG), IgM-enriched hIVIG have more advantages in the neutralization of pathogens and related toxins, the regulation of complement activity, secreti...
Detection and analysis of two drug-induced antibodies in suspicious blood samples from pre-transfusion compatibility tests
Objective To detect the piperacillin and amoxicillin antibodies in suspicious blood samples from pre-transfusion compatibility tests in Wuxi and analyze the general characteristics of them, so as to eliminate the interfe...