HCV-Core Region: Its Significance in HCV-Genotyping and Type Dependent Genomic Expression
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2012, Vol 5, Issue 1
Abstract
Present study reports the standardization and use of polymerase chain reaction (PCR) followed by RFLP (PCR-RFLP) implicating HCV-core as a target region and its comparison with PCR-RFLP based on the use of 5'NCR for HCV genotyping / sub-typing. The new assay produced 399bp PCR product from core region and used a different set of restriction enzymes (MboI, AccI and BstNI) to digest these products for HCV sub-typing. This assay allows a quick determination of HCV types / sub-types. HCV was typed / sub-typed using these two assays in patients with liver and renal diseases. Analysis of results demonstrated the two assay system to be comparable with some difference in few cases. Whereas genotype-3 was a common finding in liver disease groups, genotype-1 was more frequent in chronic renal failure cases. The PCR product from core based assays was also subjected to sequence analysis for genotypes/sub-types and the findings were slightly different. This was further analysed by phylogenetic analysis which supported our findings.
Authors and Affiliations
Mohammad Ahmad Ansari| All India Institute of Medical Sciences, Department of Laboratory MedicineClinical Biochemistry, Division Room No. 11, 2nd Floor, OPD Block, Delhi, New Delhi 110029, India, Raghavendra Lingaiah| All India Institute of Medical Sciences, Department of Laboratory MedicineClinical Biochemistry, Division Room No. 11, 2nd Floor, OPD Block, Delhi, New Delhi 110029, India, Mohammad Irshad| All India Institute of Medical Sciences, Department of Laboratory MedicineClinical Biochemistry, Division Room No. 11, 2nd Floor, OPD Block, Delhi, New Delhi 110029, India
Keywords
Related Articles
- EP ID EP8843
- DOI 10.3889/MJMS.1857-5773.2011.0208
- Views 331
- Downloads 15
Cadaver Transplantation in Balkans: Mission Possible?
Background: Donation of organs is on a very low level and due to its specific nature is a very sensitive topic, especially in the post – conflict and multinational country like Republic of Macedonia. Aim: The NGO Nat...
Jordan B. Minov. Lung and Pleural Diseases Related to Occupational Exposures. Skopje: Pristop MK & Institute for Occupational Health of Republic of Macedonia; 2009. 247 pages; ISBN 978-9989-2713-2-8; price: MKD 600
Field of medicine: respiratory medicine, occupational medicine Audience: occupational medicine specialists, internists, allergists, otorhinolaryngologists, radiologists, epidemiologists, general practitioners Purpose:...
Serum Concentration of Immunoglobulin Classes and Subclasses in Macedonian Elderly People
Aim: The aim of this study was to determine the serum concentrations of immunoglobulin classes (IgA, IgM and IgG) and subclasses (IgG1, IgG2, IgG3 and IgG4) in elderly individuals from the Republic of Macedonia. Mater...
Antibiotic Resistance Patterns of Staphylococcus aureus Isolated from Nostrils of Healthy Human Subjects in a Southeastern Nigeria Locality
Background. Antibiotic resistant bacteria have been a source of ever-increasing therapeutic problem with profound health and socioeconomic pressures. Hence, continued surveillance for bacteria susceptibility pattern is...
Multidiciplinary Treatment of a Haemophilic Person with Total Hip Replacement Surgery- A Case Report
Background: Treatment of hemophilic arthropathy may be conservative and operative. Conservative therapy includes medications and physiotherapy, and surgery includes synovectomy, tendon release, capsulotomy, osteotomy a...