Heamolytic activities, deoxyribonuclease production and in-vitro flouroquinolones susceptibility profile of aerobic gram positive cocci associated with acne vulgaris
Journal Title: Scientific Journal of Biological Sciences - Year 2012, Vol 1, Issue 2
Abstract
Bacteriological investigations were carried out on forty–six (46) swabbed samples from the pustular and nodulocystic skin lesions of undergraduate students with acne vulgaris using standard bacteriological methods. The fluoroquinolones susceptibility profiles, heamolytic activities, deoxyribonuclease production were determined using Kirby –Bauer disc diffusion methods, Columbia Blood Agar (CBA) and Deoxyribonuclease (DNase) agar, respectively. The results showed that all the 46 clinical samples showed positive growth, with 24 (52.2%) having single bacterium isolated. Co-infection with two bacterial species was seen in 24 (28.3%), while polybacterial growth was present in 9 (19.6%) of the samples. Staphylococcus aureus was the most prevalent aerobic Gram positive cocci associated with acne vulgaris with 32 (42.1%) occurrences, followed by Staphylococcus epidermidis 26 (34.2%) and Micrococcus spp 18 (23.9%). Sixteen of the isolates produced α – haemolysin, 25 (32.9%) produced β – haemolysin and 35 (46.1%) produced no haemolysin (γ-haemolysis). Of the 16 α – haemolysin producers, Staphylococcus aureus produced the highest 9 (56.3%), followed by Staphylococcus epidermidis 5 (31.3%) and Micrococcus spp 2(12.5%). Of the seventy – six aerobic Gram positive cocci isolated only 33 (43.4) were DNase producers, while 43 (56.6) were non-DNase producers. Staphylococcus aureus were highly sensitive to Moxifloxacin, while 26 (81.3%) were sensitive to Lomefloxacin and Gatifloxacin. Staphylococcus epidermidis and Micrococcus spp were highly sensitive to Gatifloxacin, Moxifloxacin, Lomefloxacin and Levofloxacin. The results also showed that 17 (65.4%) and 13 (50.0%) of Staphylococcus epidermidis were sensitive to Pefloxacin and Nalidixic, respectively. Consequently, this study has shown that fluoroquinolones could be drugs of choice to administer to patients with acne vulgaris.
Authors and Affiliations
O. J. Akinjogunla*| Department of Microbiology, Faculty of Science, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria, O. I. Medo| Department of Microbiology, Faculty of Science, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria, E. P. Philip| Department of Microbiology, Faculty of Science, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria
Keywords
Related Articles
- EP ID EP1063
- DOI -
- Views 626
- Downloads 43
Adoption of improved millet varieties by farmers in Argungu local government area of Kebbi State
The aim of this investigation was to evaluate some of the effects of aqueous leaf extract of Salvia officinalis on the kidney and liver of male Sprague Dawley rats. Ten Sprague-Dawley rats (7-11 weeks old) were randomly...
Prevalence and associated risk factors for soil transmitted Helminth infections among Urban Dwellers, Amanuel Town, Northwest Ethiopia
Infection by soil transmitted helminthes has been increasingly recognized as an important public health problem in poor and developing countries. To initiate prevention and control measures for these neglected diseases,...
Some biological aspect of carasobarbus luteus (Heckel, 1843) in hoor al-azim wetland
The Population Dynamics and status of Hemeri (Carasobarbus luteus) in Hoor Al-azim wetland (Khuzestan provinces, Iran) were investigated to derive information required for their management. During this study from 2011 to...
Study of genetic variation and phenetic relationships in some vulnerable taxa from Indian sundarbans following RAPD marker analysis
The mangrove ecosystem plays an important role in maintaining coastal ecological balance and is also one of the most highly productive and dynamic ecosystems to mankind, providing food, livelihood and ecological security...
Comparative morphologic and morphometric studies on the lower respiratory tract of adult Japanese quail (coturnix japonica) and pigeon (columbia livia)
This study was concerned with the comparative evaluation of the morphologic and morphometric parameters of the lower respiratory tract of Japanese quail and that of pigeon. In the course of this work twenty birds (ten pi...