Hepatitis C Virus E1 Protein Enhances Macrophage iNOS Expression In-vitro
Journal Title: Journal of Clinical Immunology & Microbiology - Year 2024, Vol 5, Issue 2
Abstract
Interferon-γ (IFN-γ) is a key cytokine in the adaptive immune response that is primarily secreted from CD4+ T helper cells to induce Cytotoxic T lymphocyte (CTL) cell response against HCV infection. IFN-γ activates macrophages in the liver resulting in inhibition of viral replication and increased NO production. HCV-infected macrophages are major producers of NO in the liver. It is not completely understood how HCV proteins affect iNOS expression and what the role of IFN-γ is in HCV protein expression in HCV-infected macrophages. Objective: Evaluate hepatitis C virus proteins’ regulation of IFN-γ-activated macrophage cell line. Methods: RAW-264.7 cells were seeded in 6 well-plates and transfected with HCV protein expressing plasmids using lipofectamine. After treating with IFN-γ, we determined the iNOS and HCV core, NS5A and E1 protein expression with immunoblotting. Results: Consistent with other studies, HCV core and NS5A proteins induced iNOS expression in macrophages. Moreover, HCV E1 protein-enhanced iNOS expression is highest in the presence and absence of IFN-γ activation. Conclusion: These results indicate that hepatitis C virus core, NS5A, E1 protein regulates iNOS protein expression in IFN-γ-activated and resting macrophage cell lines. These findings point to a future research direction for understanding the pathogenesis of HCV-related liver inflammation.
Authors and Affiliations
Batkhishig Munkhjargal1,2, Bilguun Enkhtuvshin1,2, Uranbileg Ulziisaikhan1,2, Baljinnyam Tuvdenjamts3, Khulan Unurbuyan1,3, Dolgorsuren Sandagdorj4, Tuul Baasandalai1, Lkhagvasuren Damdindorj5, Baasansuren Enkhjargal1, Ulziisaikhan Batmunkh1, Batbaatar Gunchin1, Tsogtsaikhan Sandag1, Bilegtsaikhan Tsolmon1, Gansukh Choijilsuren1* , Enkhsaikhan Lkhagvasuren1,6*
Keywords
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- EP ID EP740924
- DOI http://dx.doi.org/10.46889/JCIM.2024.5206
- Views 30
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