High-intensity interval training increases mitochondria biogenesis in adipose tissue and improves insulin resistance in high fat diet-induced obese rat
Journal Title: International Journal of Applied Exercise Physiology - Year 2019, Vol 8, Issue 1
Abstract
The purpose of this study was to investigate the effects of high intensity interval training (HIIT) on PGC-1α and mtDNA of subcutaneous adipose tissue and insulin resistance in obese male rats. After inducing obesity with high fat diet(for 10 weeks), eight rats from the high-fat diet group (O) and eight rats of the standard dietary group (C) were sacrificed and other obese rats were randomly divided into two groups: obesity control (OC, n=8) and high intensity interval training (HIIT, n=8). The HIIT protocol includes 10 bouts of 4-minute activity with equivalent intensity of 85-90% vo2max and 2 minute active rest periods for 12 weeks and 5 sessions per week. Western Blot method was used to measure PGC-1α and real time PCR was used to measure. Obesity induced by a high-fat diet resulted in a significant reduction in protein levels of PGC-1α [0.25±0.13 vs. 1] and insignificant reduction in gene expression of mtDNA [0.80±0.02 vs. 1]. In contrast, HIIT resulted in a significant increase in protein levels of PGC-1α [1.64±0.40 vs. 0.29±0.03] and gene expression of mtDNA [4.92±0.59 vs. 0.89±0.02]. In addition to, HIIT resulted in a significant decrease in serum levels of insulin [1.49±0.15 vs. 2.14±0.43 ng/ml] and glucose [111.16±4.07 vs. 154.66±13.21 mg/dl] and improved insulin resistance insulin [1.96±0.20 vs. 3.89±0.54]. It seems likely that HIIT result in increase of adipose tissue mitochondrial biogenesis in obesity which may be involved in improving insulin resistance.
Authors and Affiliations
Mousa Khalafi, Hamid Mohebbi, Pouran Karimi
Keywords
Related Articles
- EP ID EP572157
- DOI 10.30472/ijaep.v8i1.366
- Views 66
- Downloads 0
Does long term sport rock climbing training affect on echocardiography and heart rate variability in sedentary adults? A randomized, and controlled study.
Regular physical activity can cause some long term effects on human body. The purpose of this research was to examine the effect of sport rock climbing (SRC) training at 70 % HRmax level on echocardiography (ECHO) and he...
Effect of Training and Gender on Plasma Irisin, Leptin, and Insulin Levels
The objective of this study is the comparison of the irisin, leptin, and insulin hormone levels of the female and male elite athletes and non-athletes. Elite taekwondo athletes (7 males, 6 females) and university student...
Effects of Plyometrics and Plyometrics Combined with Dynamic Stretching on Vertical Jump in Male Collegiate Volleyball Players
Vertical jump ability is a crucial component for success in volleyball. Plyometric training is used to generate and enhance explosive power of athletes in various sport activities. The purpose of this study was to find o...
Designing the organizational creativity based on job characteristics and job involvement among physical education units’ employees of Technology University
The aim of the present study was designing the organizational creativity based on job characteristics and job involvement among physical education employees of Technology University. The statistical population of the pre...
Molecular Signals and Skeletal Muscle Adaptation to Exercise
The phenotypic plasticity of skeletal muscle affords a considerable degree of adaptability not seen in other bodily tissues. The mechanical properties of skeletal muscle are highly dependent on loading conditions. The ex...