Highlighting the Degradation of Actin in Longissimus dorsi Muscle of Different Species: Bovine, Ovine, Caprine, Poultry and Freshwater Fish
Journal Title: Biotechnology Journal International - Year 2015, Vol 7, Issue 4
Abstract
This research is aimed at highlighting the degradation of actin in the Longissimus dorsi muscle of five species, namely, bovine, ovine, caprine, poultry, and freshwater fish. To reveal the degradation of actin, by Western blotting, the myofibrillar proteins of the five studied species were first separated by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and colored with coomassie brilliant blue G-250. The colored polyacrylamide gel showed a similarity of the bands’ electrophoretic profile in bovine, ovine, and caprine. However, the bands observed in poultry and freshwater fish are specific for each species. The Western blotting revealed the presence of actin fragment of 32 kDa in bovine, ovine, and freshwater fish. A fragment of 28 kDa was found in the five studied species. However, a fragment of 19 kDa was obtained only in caprine. Two fragments of light molecular weights, 15 and 12 kDa, were found in four species such as bovine, ovine, caprine, and poultry. The fragments of 12 and 28 kDa were revealed for the first time by this research, where no previous study has announced their existence.
Authors and Affiliations
Hasina Rasolofoharitseheno, Wisdom Michael Mompi, Yasmine Boudida, Mouhammed Gagaoua
Keywords
Related Articles
- EP ID EP237188
- DOI 10.9734/BBJ/2015/17054
- Views 155
- Downloads 0
Selenium Treatment Alleviated Oxidative Alteration Generated by Cadmium in Sunflower Roots
The present study investigated the role of selenium (Se) in regulating cadmium (Cd)-induced oxidative stress in sunflower (Helianthus annuus) roots. Short-term exposure of plants to 20 µM Cd intensively increased hydroge...
Relationship between Physico-chemical Parameters and Phylogenetics Study of Human Low Density Lipoprotein Receptor-Related Protein (LRP)
In this study, 11members of human low density lipoprotein receptor-related protein (LRP) sequences was retrieved from UniProtKB/ SWISS-PROT protein database and was analyzed for information about their structural, functi...
Diversity Analysis of Some Traditional Rice Genotypes in Sri Lanka
Aims: To study the diversity of traditional rice genotypes in Sri Lanka using cluster analysis and principle component analysis. Study Design: The experiment was carried out using one hundred rice genotypes with six mod...
Production of New Almond-Peach Hybrid Rootstocks Resistance to Root-Knot Nematode
Aims: This research was conducted to hybridize almond (Om El-fahm and M. Dalet) female parent with Okinawa peach rootstock as a male parent to introduce new almond rootstock resistance to root-knot nematode. Methodology...
Investigation of Genetic Diversity among Medicago species Using RAPD Markers
Aims: Medicago is known as the Queen of forage with potential economic importance to our society. The present study aimed at the use of RAPD-PCR DNA marker to identify the genetic fingerprints affinities of six species o...