Histochemical GUS Expression of Cotton SPS Promoter in Transgenic Tobacco
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2016, Vol 8, Issue 1
Abstract
Sucrose phosphate synthase has role in synthesis of sucrose and cellulose in higher plants. A 2 kb cotton sucrose phosphate synthase (SPS) promoter and its 1.5 kb deletion fragment were cloned in plant expression vector pGA482 respectively. Both promoter fragments were transformed stably in tobacco through Agrobacterium mediated transformation. SPS promoter was found to express in tobacco plants. Full length 2 kb promoter showed high expression as compared to 1.5 kb fragment. The study revealed that SPS promoter was active in heterologous system and may be used to transform transgenes in dicots.
Authors and Affiliations
Nadia Iqbal, Ammara Masood, Aftab Bashir, Muhammad Asif
Keywords
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- EP ID EP351733
- DOI 10.9734/JABB/2016/27071
- Views 84
- Downloads 0
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