Histologic preparation of un-decalcified hard oral tissue: classic and modern methods vs. imagistic sample analysis
Journal Title: Medicine in Evolution - Year 2018, Vol 0, Issue 1
Abstract
Bone physiology has been better understood in the 1950s, with the development of plastic-embedding techniques for microscopic examination of the un-decalcified sections of bone tissue.1 In the past, bone histology required removal of its most important component, the mineral. New technologies were refined since then. This review presents classic and new methods in preparing un-decalcified bone tissue samples, especially bone-implant samples. Some practical recommendations on the histological processing of un-decalcified bone samples are presented, as well as up-to-date cutting/grinding equipments, fixation methods, embedding materials and staining techniques. Data relating to information processing using special histological techniques in comparison to micro-computed tomography are provided.
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