Histological Studies on Heteracris littoralis (Rambur) Treated with Silica Nano-particles / Challenger Formulation
Journal Title: Asian Journal of Biology - Year 2017, Vol 2, Issue 3
Abstract
Aim: Local diatomaceous earth (Silica nano-particles) and commercial pesticide (Challenger 36% SC) formulation was tested against alfalfa grasshopper, Heteracris littoralis (Rambur, 1838) (Orthopetra: Acrididae) under laboratory conditions. Study Design: Histological study for alimentary tract using light microscope. Place and Duration of Study: Department of Pests and Plant Protection, National Research Centre, Egypt- 2016-2017. Methodology: Fourth instar nymphs of H. littoralis were taken from laboratory culture reared on semi-artificial diet [1] for the experiments. The nymphs were fed on diet mixed with 1% concentration of Silica nano-particles/Challenger formulation. The tissue specimens of the alimentary canal were dissected in 0.9% NaCl solution and fixed in Bouin's solution for 24 hours [2] then dehydrated in ascending alcoholic series and cleared in Xylen for few seconds, and then specimens were infiltrated in three changes of paraffin wax each lasted 20 minutes. With Ehrlich's acid haematoxylin and alcoholic eosin. The stained sections were dehydrated, cleared and mounted using D.P.X. For microscope examination. Results: The Silica nano-particles induced great irregularity or deformity in the alimentary tract, that leading finally to death. All parts of the alimentary tract were affected by silica treatment (fore-gut, mid-gut and associated gastric caeca and hind-gut). Conclusion: Histological changes were observed as a result of Silica effect on the enzymes and the physiology of the treated insect. So, it could be concluded that Silica nano-particles may be used through the integrated pest management programme against H. littoralis by spray on the plants or as a food bait traps.
Authors and Affiliations
Aziza F. Sharaby, Amany R. Ebeid, Mohamed A. Gesraha
Keywords
Related Articles
- EP ID EP337840
- DOI 10.9734/AJOB/2017/33780
- Views 84
- Downloads 0
Assessment of Phenology and Morphological Diversity of 3 Species of Asteraceae: Anacyclus clavatus, Chamaemelum fuscatum and Tanacetum parthenium
The present paper deals with three species of Asteraceae: Anacyclus clavatus, Chamaemelum fuscatum and Tanacetum parthenium that have a wide range of uses in medicine and in industry. The detailed morphological character...
Performance of Six Faba Bean Genotypes and Their F2 Hybrids and Reciprocals
The present investigation was carried out at Giza and Sids Research Stations during 2011/2012 to 2013/14 growing seasons. A diallel cross including reciprocals among six faba bean genotypes (, Giza 843, Nubaria 1, Cairo...
Plants as Expression Systems for Recombinant Proteins
Plants provide a wide range of important biological molecules that are needed in research, medicine and industry. Currently, there is a growing interest in the use of plants as expression systems for the production of re...
Species Composition and Relative Abundance of Mosquito Larvae in Suez Canal Zone, Egypt
Mosquito-borne diseases have a great impact on human and animal health throughout the world, including Egypt. A survey was conducted at Suez Canal Zone for 8 months, prolonged from November 2014 till April 2016 to identi...
First Record of Bluntnose Sixgill Shark Hexanchus griseus (Bonnaterre, 1788) (Hexanchiformes, Hexanchidae) from Hadhramout Coast, Gulf of Aden
The new record of Hexanchus griseus (Bonnaterre, 1788) based on 148.1cm as total length was collected during the traditional fishing activities in Hadhramout coast, north east Gulf of Aden, Yemen. It is compared with the...