Human Mesenchymal Stem Cells Impregnated with Autologous Cartilage Paste Repair Fresh Focal Osteochondral Defects in Rabbits
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 4
Abstract
Introduction: Several therapies have been attempted to treat focal osteochondral defects of the knee joint, but none of them resulted in full repair. We try in this study to determine the efficacy of human umbilical cord stem cell xenografts, on the healing of focal osteochondral cartilage defects, in rabbit's knee. Material and Methods: Focal defect was created in the medial femoral condyle of the right knee of all rabbit groups. In one group the defect was left untreated. Human umbilical cord mesenchmal stem cells (MSCs) were placed in the defect, in a second group. A third group received MSCs on a fibrin sealant scaffold. A fourth group received minced cartilage paste impregnated with MSCs. Healing was assessed clinically, radiologically and pathologically. Results: The animals were able to move normally after 8 weeks of surgery. Examination of the knee joints showed better healing of the defect in rabbits that received MSCs+ cartilage paste compared to other animal groups. Magnetic resonance imaging (MRI) showed persistent osteochondral defect in rabbits treated with MSCs, without and with fibrin sealant. Complete defect fill, intact cartilage surface, was observed in rabbits treated with MSCs impregnated with cartilage paste. Pathologic examination revealed that MSCs, alone, induced chondrocyte proliferation and stimulated cartilage repair. A better result was obtained with MSCs + fibrin sealant. The best repair was obtained when autologous cartilage paste was used. Conclusions: Repair of focal osteochondral defects in rabbit knees using human umbilical cord MSCs impregnated with autologous cartilage paste appears to be successful as proven clinically, radiologically, as well as pathologically.
Authors and Affiliations
Hazem Mahmoud Atta, Mohammad Mohammedaly Abbas, Mohammad Hussein Alkaf, Mamdouh M Kotb, Laila A Damiati, Gamal Karrouf, Soheir Mahfouz
Keywords
Related Articles
- EP ID EP546728
- DOI 10.21276/ijmrp.2017.3.4.021
- Views 110
- Downloads 0
Evaluation of Jeddah Governmental Health Institutes in Health Preparedness to Chemical and Biological Weapons Of Mass Destruction
Introduction: Terrorism has become recognized widely as a significant threat to the safety and public as a result of the rise of technical capabilities, the rapid expansion of the global biotechnology industry, and the g...
Importance of Duodenal Bulb Biopsy in Pediatric Patients for Diagnosis of Celiac Disease
Background: Celiac disease (CD) is a gluten-dependent enteropathy. It is a chronic gastrointestinal disorder in which ingestion of gluten, a protein present in wheat, rye and barley leads to damage of the small intestina...
Students' Perception of Pharmacology and Teaching - Learning (T-L) Methods Used in Pharmacology
Introduction: Sound knowledge of Pharmacology plays a significant role in safe and rational use of drugs in clinical practice but the subject is difficult to understand and retain. Moreover many new drugs are introduced...
Efficacy of CPAP in Treatment of Hyaline Membrane Disease in NICU
Hyaline membrane disease also called as respiratory is a common cause of admission in the NICU. It is a common health problem in preterm babies due to deficiency of surfactant. Surfactant reduces surface tension in the a...
Early Development of Overt Proteinuria in Extremely Uncontrolled Type 1 Diabetic Patients
Introduction: Overt proteinuria usually occurs in 15 to 40% of patients with type1 diabetes, with a peak incidence around 15 to 20 years of diabetes. The aim of the study is to know the incidence of overt proteinuria in...