Human Papilloma Virus 16 and 18 Association in Cervical Intraepithelial Lesions and Cervical Cancers by In Situ Hybridization
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2017, Vol 6, Issue 3
Abstract
Objective: To correlate the association of high risk Human Papilloma Virus (HPV) 16, 18 in cervical intraepithelial lesions and cervical cancers by in-situ hybridization (ISH) technique. Study Group: Cervical biopsy and hysterectomy specimen of 78 young and adult women, attending Hi-Tech Medical College and Hospital, Bhubaneswar, who were clinically or cytologically suspected of cervical intraepithelial lesion or cervical cancer were taken as source of target viral DNA. Material: Formalin 10% as fxative H & E stain as routine staining agent In-situ hybridization kit for HPV 16 and 18 DNA. Method: After following standard protocol for surgical grossing, HPV 16, 18 In-situ hybridization kit was used on paraffn embedded tissue sections. Results: The percentage of positive cases was highest in cervical cancer patients followed by cervical intraepithelial lesions, high grade, and low grade. Conclusion: This study has been carried out for the frst in our state and our results show high degree of positivity of HPV 16/18 in females with cervical intraepithelial lesions and cervical cancers attending our tertiary care hospital.
Authors and Affiliations
Mohanty Manisa| Department of Pathology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India, Corresponding e-mail: mohantymanisa@gmail.com, Mohanty Ramesh Chandra| Department of Pathology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India, Mohanty Raghumani| Department of Pathology, Hi-Tech Medical College and Hospital, Bhubaneswar, Odisha, India, Swain Rajeeb| Institute of Life Sciences, Bhubaneswar, Odisha, India
Keywords
Related Articles
- EP ID EP12327
- DOI -
- Views 290
- Downloads 14
Influence of Altiazem PP-180 on Hemodynamic Parameters in Arterial Hypertension Patients under Outpatient Conditions
The aim of this research work is to study the effect of altiazem PP-180 on hemodynamic parameters in 78 II-degree arterial hypertension patients under outpatient conditions. Hemodynamic effect of altiazem PP-180 hypoten...
ASSESSMENT OF SATISFACTION IN PERITONEAL EQUILIBRATION TEST: A STUDY ON THE VALIDITY AND RELIABILITY OF THE PERITONEAL EQUILIBRATION SATISFACTION SCALE
Aim: This study has been designed to develop an assessment tool to be used in determining the patients’ satisfaction level with the peritoneal equilibration test (PET) procedure. Materials and Methods: The developme...
The effect of telephone follow-up after ambulatory surgery on pain management for children at home by parents
Since time was short hospitalization after ambulatory surgery after discharge the duty of care of children at home, and parents are responsible, their familiarity with pharmacological and nonpharmacological methods of p...
Investigating the Effect of Teacher Training Program on Understanding Teachers’ Supportive Behaviors by Students with Diabetes
Introduction: insufficient information of teachers on diabetes lead to emergence of many problems for pupils. Therefore, this research aimed to investigate the effect of teacher training program on perception of teacher...
REPARATIVE OSTEOGENESIS DURING TREATMENT OF FRACTURE UNDER TRANSOSSEOUS OSTEOSYNTHESIS AND INTRAMEDULLARY INSERTION OF WIRES WITH HYDROXYAPATITE COATING
Background: The problem of improving medical care for patients with the locomotor system injuries is very important especially last time. Material and Methods: Canine open comminuted tibial fractures modelled experimen...