HYDROALCOHOLIC EXTRACTION OF SHOOT CULTURES FROM NOTHAPODYTE NIMMONIANA AND ITS ANTI-PROLIFERATIVE ANALYSIS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 6
Abstract
Objective: The important restraints in plant-derived medicine are the convenience of active composites which depends on diverse topographical situations, active compound build-up pattern, environmental circumstances and genetic makeup of the plant. The presented work was aimed to describe an efficient technique for fast propagation of Nothapodytes nimmoniana which is an imperative source of Camptothecin.Methods: Multiple shoots were successfully regenerated from the explants N. nimmoniana using Murashige and Skoog (MS) medium accompanied with IBA+KN+GA3 (1:3:2) mg/l. the hydro-alcoholic extract was obtained by soxhlet extraction for field grown roots (fgRE), shoots (fgSE) and in vitro plantlets (ivPL) and further subjected to phytochemicals analysis. HPLC analysis was performed to determine the percentage of camptothecin (CAM). Anti-proliferative studies were carried out followed by Acridine orange/Ethidium bromide staining. Extracts were also estimated for reactive nitrogen species (RNS) and reactive oxygen species (ROS).Results: Preliminary qualitative chemical analysis of the extracts displayed the presence of alkaloids, carbohydrates, steroids, saponins, terpenoids and phenolics compounds. HPLC analysis revealed the presence of CAM in all the extracts. ivPL exhibited lowest anti-proliferative activity in contrast to other extract and percentage of ROS and RNS was also less. Although ivPL did not exhibited prominent anti-tumour effect, but in vitro accumulation of CAM in 2-months old shoots provides an alternative resource to 5 y-old field grown plants.Conclusion: This work delivers a potential to lead to further increase the potency of CAM in in vitro shoots of two months old as an alternative to the field grown plants for treating cancers.
Authors and Affiliations
Arun Sharma, Ankita Rajata, Udayabanu Malairaman, Hemant Sood
Keywords
Related Articles
- EP ID EP567467
- DOI 10.22159/ijpps.2018v10i6.25726
- Views 84
- Downloads 0
BRINE SHRIMP LETHALITY BIOASSAY OF ABRUS PRECATORIUS (LINN) LEAVES AND ROOT EXTRACT
Objective: The present study was conducted to test for in vivo Brine Shrimp Lethality Assay (BSLA) of Abrus precarious leaves and root extracts after successive maceration in four solvents (n-hexane, dichloromethane (DCM...
STUDY ON THE INTERACTION OF AMITRIPTYLINE HYDROCHLORIDE AND MALONIC ACID BY SPECTROPHOTOMETRIC AND SPECTROFLUORIMETRIC METHODS IN TABLETS: APPLICATION TO CONTENT UNIFORMITY TESTING
Objective: The objective of this research to study the interaction between amitriptyline hydrochloride in drug products via reaction with mixed acids anhydrides: Application to Content Uniformity Testing.Methods: Malonic...
CHEMICAL COMPOSITIONS, ï¡-AMYLASE INHIBITORY AND ANTIOXIDANT ACTIVITIES OF THE ESSENTIAL OILS FROM UNRIPE FRUIT PULP AND LEAVES OF SYZYGIUM CUMINI
Objective: To investigate chemical compositions, a-amylase inhibitory and antioxidant capacity of the essential oils from the unripe fruit pulp and leaves of a traditional medicinal plant Syzygium cumini.Methods: The ess...
PREVALENCE OF HUMAN PAPILLOMA VIRUS TYPES 16 AND 18 AMONG WOMEN WITH CERVICAL CANCER IN ALEPPO, SYRIA
Objective: Cervical cancer is the second common type of cancer among women worldwide. Infection by certain types of human papillomavirus infection (HPV) such as HPV 16/18 has been considered as the most significant risk...
THE ROLE OF HONEY WITH ROYAL JELLY IN PROTECTING THE GRAAFIAN FOLLICLES FROM THE TOXICITY OF THE ADRIAMYCIN DRUG
Objective: This study aims to investigate the ability of certain mixture of honey with royal jelly (H+R) to prevent the toxic side effects, caused by the Adriamycin drug. One of the most important side effects of this dr...