IDENTIFICATION ACTIVE COMPOUNDS OF BACTERIA STREPTOMYCES USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2017, Vol 3, Issue 6
Abstract
Aim of the study assess optimum Growth of Streptomyces and identification active compounds of bacteria Streptomyces using HPLC. The biochemical and physiological testes of Streptomyces sp. Show that the Streptomyces produced extra cellular enzymes like amylase, urease, catalase, lipase, protease, pectinase, cellulase and phosphatase, no HCN production and negative for indole production. Estimating the stationary phase for isolated Streptomyces were growing on malt extract yeast extract broth ( ISP2), and getting the optical density (OD) at 600nm during (1-7) days and optimal growth of them ranged (6.5 to 8), while were salt concentrations ranged from (1 to 7% NaCl). The best growth happened at 30 °C by measuring the absorbance of the growth and getting the optical density (OD) at 600nm. We watched the growth state activity of isolated strain which started after 72 hours of incubation at 30°C, when the OD was about (0.572 ). The HPLC analysis of extracellular crude extract was showed found five different antibiotic (Tetracycline, Streptomycin, Neomycin, Vancomycin and Kanamycin).
Authors and Affiliations
Dr. Mohsen Hashim Risan
Keywords
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