Identification and cloning of PIP2-like gene in Poncirus trifoliata and Swingle citrumelo
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 7, Issue 1
Abstract
Water transport through cellular membranes is facilitating by water channels, the so-called aquaporins. Aquaporins were shown to be involved in many physiological processes such as root water uptake, reproduction or photosynthesis. There are some evidences confirming that PIP proteins could regulate the whole water transport through plant tissues. This study is aimed to isolate the PIP2 gene in Poncirus trifoliata and Swingle citromelo. The fragment of this gene was amplified by polymerase chain reaction and then was cloned in a TA vector. The sequence of PIP2 gene exhibited a high similarity with the other plant PIP2 genes in the NCBI gene bank. The results indicate that this gene sequence owns a higher identity (97%) with Citrus sinensis PIP2-1-like gene that belongs to the same taxonomic family with trifoliate orange (Rutaceae).
Authors and Affiliations
M. Yusefi, M. R. Dadpoor, A. Movafeghi, F. Zaree Nahandi, D. Zare Haghi, M. Kosari-Nasab
Keywords
Related Articles
- EP ID EP38563
- DOI -
- Views 159
- Downloads 0
Influence of earthworms and humic acid on some microbial indices in a Pb contaminated soil
Increasing human population pressure has decreased the availability of arable land and it is no longer feasible to use extended fallow periods to restore soil fertility. The fallow period which would have restored soil...
Molecular genotyping of methicillin-resistant Staphylococcus aureus by employing DNA marker technology
Staphylococcus aureus are Gram positive, non-motile and facultative anaerobic coccus often golden yellow cells with pigments and usually arranged in clusters like grapes. Methicillin-Resistant Staphylococcus aureus (MRSA...
Sustainable architecture and urbanization based on a stable environment
One of the greatest concerns of the international community is destruction of sources of energy. Architecture and urbanization as efficient and effective factors in conserving this resource can contribute to survival of...
Level of Arsenic in Ground Water and Its Impact on Human Health: A Case Study of Bahawalpur City, Pakistan
Access to clean and pure drinking water is basic human right. Poor drinking water quality causes many diseases including Cancer (Lung, Liver, Skin, and Kidney Cancer). The major goals of this study are to measure level o...
Species/geographic boundaries and evolutionary interrelationships of cultivated linden-trees (Tilia L.) based on morphological and nrDNA ITS characteristics
Nuclear ribosomal transcribed spacers (ITS1-5.8S rRNA-ITS2) is a popular marker that has superior taxa resolution in some groups of organisms. A taxonomic reassessment of 27 Tilia taxa combining a molecular marker (ITS...