Identification of endophytic fungi from the medicinal plants of Biligirirangana hill, Karnataka
Journal Title: Journal of Applied and Natural Science - Year 2018, Vol 10, Issue 4
Abstract
Four plants with medicinal properties Carica papaya, Phyllanthus amarus, Tinospora cordifolia and Azadiracta indica were selected as a host plant for the isolation of endo-phytic fungi collected from Biligirirangana Hill, ranges from 11° 40’ to 12° 09’ North and 77° 05’ to 77° 01’ East, the southern part of Eastern Ghats Chamarajanagar District, Kar-nataka. The leaves of these plants revealed two, four, six and three isolates of endophytic fungi which belonged to the family Hypomycetes, Ascomycetes, Dothideomycetes, Coleo-mycetes respectively. One of the unidentified strain from each of the above four medicinal plant isolate was subjected to molecular analysis followed by 18SrRNA sequence, inter-nal transcribed spacer regions and phylogenetic analysis. Based on the gene sequencing and phylogenetic tree the clusters species of the fungal isolate was found to be Stem-phylium lycopersici, Epicoccum nigrum, Leptosphaerulina arachidicola and Phomopsis azadirachtae reported in this paper.
Authors and Affiliations
K S Sushma, M Jayashankar, A K Vinu, Mohammed Ali Saeed
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