Identification Of Potato Breeding Lines With Tolerance To In Vitro Hydric Stress
Journal Title: International Journal of engineering Research and Applications - Year 2018, Vol 8, Issue 10
Abstract
Potato breeders from around the world, try to create genotypes with high yield and stress tolerance. In this study it was research tolerance of hydric stress for 27 genotypes by in vitro selection technique. This is based on in vitro tissue culture growth, on medium supplemented with an osmotic agent, allowing selection and regeneration of plants with desired characteristics. To induce in vitro hydric stress was used polyethylene glycol (with 2 concentration 1% and 2%) in nutritive medium and this was compared with basic medium MurashigeSkoog, considered control. Determinations were performed 4 weeks after inoculation of minicuttings belonging to 27 potato breeding lines of plantlets. The parameters analyzed were next: number of leaves, height of plantlets, root length. From obtained results, 1893/5 line can be considered as a genotype capable of extracting the water reserve and finally capable to fight with the effects of drought, so, able to resist in vitro hydric stress conditions. 1893/5 genotype was distinguished by the formation of a high number of leaves/plantlet on the medium with 2% PEG (9.17 leaves). 1893/5 genotype was representative in production of long roots, by using 2% PEG (9.9 cm).
Authors and Affiliations
Ștefan Maria, Tican Andreea, Bădărău Carmen Liliana, Prodan Delia
Keywords
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- EP ID EP401967
- DOI 10.9790/9622-0810015257.
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