Identification of Salmonella sps. from contaminated meat samples by multiplex pcr-based assay
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2016, Vol 4, Issue 0
Abstract
Poultry and cattle are the principal hosts of pathogenic bacterium Salmonella species. Therefore, development and use of a precise and sensitive method for identifying this pathogen is one of the main concerns for researchers. In this study, total 1100 cattle and poultry meat samples were evaluated to examine the presence of Salmonella by using three methods viz pre-enrichment culture, medium culture and polymerase chain reaction. Among the collected 850 cattle and 250 poultry samples 4.35% and 5.6% of the cattle and poultry samples were contaminated by Salmonella respectively. Further, among the 51 Salmonella contaminated samples of cattle and poultry, 25% were infected by S. enteritidis, 35% by S. typhimurium and 17% by S. infantis. Among the various tested methods, combination of a conventional PCR technology and standard culture medium could be effective in providing a more and accurate profile of the prevalence of this pathogen in poultry and cattle meat.
Authors and Affiliations
Behzad Nikbakht, Ali Mohamadi Sani
Keywords
Related Articles
- EP ID EP429684
- DOI 10.18006/2016.4(5).512.518
- Views 60
- Downloads 0
PERFORMANCE OF RICE GENOTYPES UNDER DIFFERENT SEED RATES AND MICRONUTRIENTS IN DIRECT SEEDED CONDITION UNDER TUNGABHADRA IRRIGATION COMMAND
The present investigation was conducted during kharif season of 2015 and 2016 at agriculture research station, Dhadesugur, University of agricultural sciences, Raichur to optimizing the performance of genotypes to differ...
Effect of nitrogen and sulfur on the quality of the cotton fiber under mediterranean conditions
Agronomic practices significantly influence the productivity and quality of cotton plant. Present study was undertaken to evaluate the effect of nitrogen and sulfur fertilizer application on the fiber quality of cotton,...
Effect of benzyladenine and salicylic acid on biochemical traits of two cultivars of carnation
Plant growth regulators have significant effect on the delaying senescence in buds and flowers. Present study was conducted to find out the effects of Salicylic acid and Benzyl adenine spraying on various biochemical tra...
PESTICIDAL REPERCUSSIONS OF DIFFERENT PLANTS OIL ON LARVAE AND ADULT of Tribolium confusum JAQUELIN DU VAL
Continues use of synthetic insecticides has resulted in resistance towards insecticides. Therefore, present study was conducted to evaluate the biocontrol potential of three essential oil fumigation viz., Zea mays, Zingi...
Effect of Morinda citrifolia in growth, production and immunomodulatory properties in livestock and poultry: a review
Morinda citrifolia L. is commonly known as Noni and has been found to have wide range of medicinal properties. It is usually found in the coastal region in many countries including Andaman and Nicobar Islands and belongs...