Identification of sibling species in field collected Anopheles minimus using ovarian nurse cell chromosome in two malaria endemic areas in Myanmar
Journal Title: JOURNAL OF BIOLOGICAL ENGINEERING RESEARCH AND REVIEW - Year 2017, Vol 4, Issue 1
Abstract
Malaria remains a major health problem in Myanmar. Anopheles dirus and Anopheles minimus are the principal vectors of malaria, but Anopheles maculatus, Anopheles vagus, Anopheles annularis, Anopheles philippnensis, Anopheles stiphensi and Anopheles culicifacies are secondary or suspected vectors of malaria in Myanmar. The aim of the present study was to know the prevalence of sibling species complex of Anopheles minimus using ovarian nurse cell polytene chromosome method and determine the potential vector using vector incrimination. Therefore the study was done in Pyin Oo Lwin Township Mandalay Region and Kamamaung Township Kayin State from October 2014 to September 2015. Anopheles mosquitoes were collected by animal bait K-net, human bait indoor and outdoor and light trap collection. A total of 1153 Anopheles mosquitoes comprising six mosquito species were collected from Pho Ne Dun Village, Pyin Oo Lwin Township. The highest number 553 of Anopheles minimus were collected. 953 Anopheles mosquitoes (524 from Katine Htit and 429 from Kine Taw) comprising nine species were collected from Kamamaung Township. Of these 93 and 92, Anopheles minimus was collected from Katine Htit and Kine Taw villages respectively. After identification blood fat Anopheles minimus was kept in paper cups with glucose. When ovary development reached to the semigravid stage (Christophe stage), ovary specimens were dissected and preserved in Cornoy’s fixative in screw type bottle and ovaries were tapered to vectors polytene chromosome. Head and thorax were homogenized tested for circumsporozoite antigen by ELISA method. Result found that all collected Anopheles minimus were observed sibling species A in both areas. Circumsporozoite ELISA test found that only one Anopheles minimus A was found sporozoite positive in Kamamaung Township 0.54% (1/185) and not found any sporozoite positivity in Anopheles minimus A collected from Pyin Oo Lwin Township (0/553).
Authors and Affiliations
Maung Maung Mya, Sein Thaung, Phyo Aung Naing, Ye Ye Myint, Thu Zar Nyein Mu, Yan Naung Maung Maung
Keywords
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