[i]Ex Situ[/i] and [i]In Vitro [/i]Conservation of [i]Glicyrrhiza glabra[/i] L. – Crop Wild Relative from Fabaceae
Journal Title: Ecologia Balkanica - Year 2014, Vol 5, Issue 0
Abstract
[i]Glycyrrhiza glabra[/i] L. (Licorice), belongs to the Fabaceae family. The species is included in the Crop Wild relatives list for Bulgaria and is close to beans and peas. Its origin is Southeast Europe, the Mediterranean and Asia, where Bulgaria falls. The roots of the plant contain glycyrrhizin, 30 to 50 times sweetener than the sucrose. As a CWR, as well as due to the high level of glycyrrhizin in the roots, the conservation and maintenance of the species is of interest, although it is not included in the list of threatened plant species yet. In the Institute of Plant Genetic Resources - Sadovo [i]Glycyrrhiza glabra[/i] is maintained in situ in the Botanical garden. Besides the in situ conservation of the species, in vitro techniques are a reliable means of reproduction and long-term storage. After introduction of the raw cuttings from plant species in culture in vitro, the process of micropropagation is accomplished by single bud microcuttings in nutrient medium fitted with growth regulators, enabling the development of single-rooted stems with options of repeatedly subcultivating. Along with that the possibility for long term in vitro propagation by reduction of the composition of the nutrient medium was tested, where the period for conservation of the cultivated explants reaches 8 months.
Authors and Affiliations
Katya Uzundzhalieva, Ruska Ruseva, Svetla Kachakova
Keywords
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