IL-8 mRNA and Serum Levels in Vitiligo Patients: A Case Control Study
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 11
Abstract
Introduction: Many theories are suggested to explain aetiology of vitiligo, such as autoimmune, genetic and biochemical. Interleukin-8 (IL-8) is a pro-inflammatory chemokine which is evaluated in the pathogenesis of some skin diseases, like psoriasis, however, in vitiligo, few studies were reported regarding this issue. Aim: The aim of the present study was to investigate serum level of IL-8 and IL-8 mRNA in patients with different types of vitiligo to validate its role in aetiopathogenesis of vitiligo. Materials and Methods: This case-control study was conducted on 39 vitiligo patients and 15 age and gender matched healthy controls. The participants were selected from Dermatology Outpatient Clinic, Menoufia University Hospitals from October 2014 to October 2015. They were subjected to clinical history, examination and full general examination. Detailed dermatological examination including VASI score was applied. IL-8 serum level was measured by ELISA and IL-8 mRNA was quantitated by Real Time-PCR. The statistical analysis was done using SPSS, version 20.0. Mann Whitney U test was used to compare two quantitative not normally distributed. Chi-square test (χ2 ) was used to study association between two qualitative variables. Spearman correlation test was applied to assess correlation between two continuous quantitative variables. A p≤0.05 was considered significant. Results: IL-8 serum level and mRNA concentration mean values were significantly elevated in vitiligo patients (26.25±43.28 pg/ mL and 8.48±11.92 ng/mL) than controls (0.57±0.50 pg/mL and 0.60±0.32 ng/mL) (p=0.002, for both), and they showed significant positive correlation with each other (r=0.622; p≤0.001). Moreover, both of them revealed significant high values in localised vitiligo (62.20±74.39 pg/mL and 12.42±13.85 ng/mL) than generalised (18.39±29.49 pg/mL and 7.61±11.52 ng/mL) (p≤0.000 and p=0.004), respectively. Conclusion: Serum IL-8 chemokine and its mRNA increased significantly in vitiligo patients indicating that it may have a dynamic role in vitiligo development and participate in its pathogenesis.
Authors and Affiliations
Azza Gaber Farag, Alaa Hassan Marae, Mona Salah Eldin Habib, Reda Zakaria Mahfouz, Nada Farag Elnaidany, Salwa Ibrahim Youssef, Sherifa Morsy El-Feky
Keywords
Related Articles
- EP ID EP366990
- DOI 10.7860/JCDR/2017/30392.10857
- Views 59
- Downloads 0
Efficacy of Combined Interscalene Block and Superficial Cervical Plexus Block for Surgeries of the Clavicle: A Prospective Observational Study
ABSTRACT Introduction: Although there are regional anaesthetic techniques used to provide analgesia in fractures of clavicle, their routine use as a sole anaesthetic technique for surgical fixation of the same has not be...
Aeroallergen Sensitivity Pattern in Poorly Controlled Asthmatics and its Relation to Asthma Control in a Tertiary Care Setting of Central Kerala, India
ABSTRACT Introduction: Nasobronchial allergy is an exceedingly common problem in clinical practice. Estimates reveal that the prevalence of nasobronchial allergy is increasing worldwide, including in India. Sensitivity t...
Heart Disease in Pregnancy-Evaluation of Spectrum, Association of Predictors with Obstetric Outcome and Need for Comprehensive Medical Care
ABSTRACT Introduction: Cardiac disorders of varying severity complicate nearly 1% of pregnancies and are an important cause of maternal morbidity and mortality. It is important to define the risk stratification of women...
Factors Predicting Outcome of Trial without Catheter in Patients with Acute Urinary Retention Secondary to Prostatic Enlargement
ABSTRACT Introduction: Acute Urinary Retention (AUR) is an important feature of progression of Benign Prostatic Hyperplasia (BPH). The management of AUR is immediate urethral catheterisation followed by Trial without Cat...
Comparison of the Effect of PRP, PRF and Induced Bleeding in the Revascularization of Teeth with Necrotic Pulp and Open Apex: A Triple Blind Randomized Clinical Trial
Introduction: Treatment of a tooth with necrotic pulp and open apex is a special challenge to the clinicians. Apexification with calcium hydroxide and MTA barrier technique fails to induce continued root maturation which...