Imidacloprid Induced Intoxication and its Biodegradation by Soil Isolate Bacillus weihenstephanensis
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 9
Abstract
Aims: Study was carried out to investigate the effect of imidacloprid on biochemical parameters and growth of soil isolate. The imidacloprid degradation by the soil isolate was also studied. Study Design: The soil isolate was identified and used for toxicity testing. The isolate of Bacillus weihenstephanensis was further tested for its ability to degrade imidacloprid in minimal salt medium (MSM) and tryptic soya medium (TSB). The role of plasmid in imidacloprid degradation was established by curing experiments. Place and Duration of Study: Department of Biotechnology and Microbiology, Karnatak University, Dharwad, India between June 2011 and December 2012. Methodology: The soil isolate was identified by morphological, biochemical characters and 16s rDNA identification. Effect of imidacloprid on DNA, RNA, protein, glucose and growth in soil isolate was studied with 10-3 to 10-7 molar imidacloprid for 96 h. Imidacloprid degradation was determined in MSM and TSB for 28 days with samples taken on 7, 14, 21 and 28th day. The insecticide concentration was tested by HPLC. Plasmid curing was performed. Results: The soil isolate was identified as Bacillus weihenstephanensis. The study involving soil isolate Bacillus weihenstephanensis with 10-3 to 10-7 molar imidacloprid showed significant (P<0.05) decrease in content of DNA, RNA, protein, glucose and growth. Bacillus weihenstephanensis in MSM and TSB showed 46 and 78 % imidacloprid degradation in four weeks. The plasmid of Bacillus weihenstephanensis was cured in fourth generation. 18.80% and 75% degradation observed in cured and non cure cells of Bacillus weihenstephanensis in TSB. Conclusion: Study showed that imidacloprid affects the biochemical contents and intern growth of soil isolate Bacillus weihenstephanensis. Study also revealed that Bacillus weihenstephanensis was able to degrade imidacloprid in MSM and TSB. Further plasmid curing revealed that the genes for imidacloprid degradation are located both in plasmid and chromosome.
Authors and Affiliations
B. B. Kaliwal, A. A. Shetti, R. B. Kaliwal
Keywords
Related Articles
- EP ID EP237718
- DOI 10.9734/BBJ/2014/8255
- Views 108
- Downloads 0
Genetic Variability in Cultured and Wild Population of Clarias gariepinus Using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Aim: The present study was undertaken to determine the genetic differences between cultured and wild populations of Clarias gariepinus in Southwest Nigeria using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis...
Evaluation of Halobacterial Extracellular Hydrolytic Activities in Several Natural Saline and Hypersaline Lakes from Romania
This work presents the evaluation of extracellular hydrolytic activities involved in the degrading of organic compounds from several natural salt lakes located in the southern part of Romania. The paper approaches the ex...
Relationship between Neutral Invertase Activity and Sugar Contents in Tomato Fruit and Its Functional Prediction Analysis
Aims: Neutral invertase (NI) probably plays an important role in sucrose metabolism of tomato, but main function and mechanism are unclear. In this study, contents of soluble sugar and NI activity were measured at differ...
Effects of Input Variables on the Conversion of 5 Ton/h Processed Tomato Juice in a Triple-Effect Evaporator
Tomato is grown in many regions of the world; and processed tomato products account for 80% of total tomato consumption. A worldwide tomato processing industry has since evolved with a range of technologies which are abl...
Assessment of Toxic Metals in Dairy Milk and Animal Feed in Peshawar, Pakistan
Aims: To assess the concentration of toxic heavy metals [Cadmium, Copper, Lead and Nickle] in dairy milk and animal feed of urban and rural areas and correlate them. Study Design: It was a cross-sectional comparative stu...