Immune Profiling of Grain Adapted I-2 (GAI-2) Pelleted Feed Vaccine in 6weeks Old Cockerels in Vom Nigeria
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
In this study, a modified wet harvest of NDVI-2 virus strain with an EID50 of 9.0/ml and a thermostability of 6 hours at 56o C was adsorbed to heat treated Digitalia iburua which served as the virus carrier and was used to prepare pelleted feed vaccine. In this trial, 100 (six weeks old) cockerels were divided into five groups of twenty each. They all were vaccinated against other diseases except Newcastle disease (ND). Group 1 was not given the pelleted feed vaccine and served as the control, while groups 2, 3, and 4 were feed with 10, 15, and 20 grams per birds respectively while group 5 was treated with the conventional NDVI-2 through the intra ocular route. Groups 2, 3 and 4 were given booster doses at different intervals. The detectible antibody was analysed using haemagglutination inhibition (HI) test. The geometric mean titre of protective antibody response in cockerel vaccinated with GAI-2 pelleted feed vaccine compared favourably with the conventional NDVI-2. Groups 2 to 5 were later challenged with wild virulent field virus (Kudu 113 ND strain) and the antibody response was astronomical, with group 2 recording the highest geometric mean titre of 2352.5 post challenge. Thus, the GA I-2 pelleted feed vaccine could be an excellent alternative to the conventional vaccination method, since the stress associated with handling of individual birds will be greatly minimized. The significance of the rapid increase in the antibody titre when challenged with wild virus strain is discussed.
Authors and Affiliations
Anthony N Egbuji, John O. Ibu, Ismaila A. Shittu, Dauda G. Bwala, Anne. S. Lohlum, Josephine Kiagama, Evelyn D. Dung, Enurah L. Uwanibe, Fatima B Mukaila, Dung S Lokason , Chinemerem. R Obene, Choji A Kwanga, Nicodemus M. Useh, Paul A Abdul, Usman Musa, John A. Okoye, Jude S. Rabo
Keywords
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- EP ID EP428127
- DOI 10.18006/2017.5(5).599.605
- Views 80
- Downloads 0
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