Immuno-Histochemical and Quantitative Study of Melanocytes and Melanin Granules in Oral Epithelial Dysplasia
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 7
Abstract
Introduction: Oral Epithelial dysplasia (OED) is a potentially malignant disorder that is characterized by the presence of architectural and cytological changes. One of the prime factors responsible for the development of these lesions is the usage of tobacco. A variety of factors provide protective mechanism in order to prevent the effects of chemotoxic agents including tobacco products of which, melanin pigmentation is one of the vital elements. Aim: Role of melanocytes in progression of OED has remained unclear, so the present study was done to evaluate density of melanocyte and melanin granules in different grades of epithelial dysplasia and to correlate both findings with different grades of epithelial dysplasia. Materials and Methods: The study included 60 OED cases, of which three histopathogical sections were prepared from each block. The sections were stained with Hematoxylin and Eosin, Masson Fontana and Human Melanoma Black (HMB45), an immunohistochemical stain. Quantification of melanin granules was evaluated under 40X magnification using arbitrary scale with micrometer square as, 0= Absence of melanin granules, 1= Rare and scattered melanin granules, 2= Dense but not aggregated melanin granules, 3= Dense and aggregated melanin granules. Density of melanocytes was evaluated under 10X magnification. Five consecutive fields were evaluated for melanocytes and melanin granules starting from the field of highest density. Results: There was an insignificant increase in number of melanocytes and melanin granules in mild and moderate dysplasia compared to normal but significant reduction was observed in severe dysplasia. Conclusion: The decrease in number of melanocytes and melanin granules was proportional to severity of epithelial dysplasia. This could be due to chronic irritation by chemical products leading to death of melanocytes.
Authors and Affiliations
Swapna honwad, sv Ravi, Mandana Donoghue, Manjiri joshi
Keywords
Related Articles
- EP ID EP357669
- DOI 10.7860/JCDR/2017/26096.10206
- Views 100
- Downloads 0
Social Security Measures for Elderly Population in Delhi, India: Awareness, Utilization and Barriers
Introduction: World population of elderly is increasing at a fast pace. The number of elderly in India has increased by 54.77% in the last 15 years. A number of social security measures have been taken by Indian governme...
Central Variant of Posterior Reversible Encephalopathy Syndrome – A Rare Case Report
Posterior Reversible Encephalopathy Syndrome (PRES) is a clinicoradiological condition associated with headache, altered mental status, dizziness and white matter vasogenic oedema in parieto-occipital region. It is often...
Evaluation of Liver Biopsies using Histopathological Scoring System in Neonatal Hepatitis and Biliary Atresia: Correlation with Clinico-Radiological and Biochemical Parameters
ABSTRACT Introduction: Biliary Atresia (BA) and Neonatal Hepatitis (NH) are common causes of Neonatal Cholestasis (NC). There is a high degree of overlap between clinical and other investigational characteristics of BA...
Burkholderia Infections in Diabetic Patients Emerging as a Challenge for Physicians: A Case Series
A BSTRACT The genus Burkholderia comprises more than 40 species. Medically important members of this genus are Burkholderia cepacia (B.cepacia), Burkholderia pseudomallei (B.pseudomallei) and Burkholderia mallei (B.malle...
Low Level Laser as a Therapeutic Alternative in Treatment of Oral Lichen Planus- Review of Literature
Abstract Oral Lichen Planus (OLP) is a chronic mucocutaneous disease having oral and systemic manifestations. Topical Steroids are the first line of treatment in OLP. Adjuncts like retinoids, placental extracts, immunomo...