IMMUNOMODULATORY ACTIVITY OF AN ACETONE EXTRACT OF TERMINALIA BELLERICA ROXB FRUIT ON THE MOUSE IMMUNE RESPONSE IN VITRO
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 11
Abstract
Objectives: To investigate the immunomodulatory activity of an acetone extract of T. bellerica fruit.Methods: Mitogen induced-lymphocyte proliferation using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) technique, Th1- and Th2-related cytokine production by lymphocytes using ELISA and peritoneal macrophage function in ICR mice were assayed.Results: The results show that the extract had a mild inhibitory effect on the generation of oxidase enzyme (Phagocytic Index 0.8, 100 mg/ml) but did not influence acid phosphatase enzyme function during phagocytosis. The extract stimulated the proliferation of both T and B lymphocytes. The maximal activation (Stimulation Index 3.2, 100 mg/ml) was presented with concanavalin A induction, indicating a major effect on T lymphocyte proliferation. The extract reduced the production of IFN-γ (89%, 100 mg/ml) and IL-2 (98%, 100 mg/ml) but increased IL-10 secretion (231%, 100 mg/ml) compared to concanavalin A. Gallic acid, a pharmacological component contained in this plant, presented a similar effect as that of T. bellerica extract and may contribute to the immunomodulatory activity of T. bellerica fruits in cooperation with other phytocompounds. The decrease in the IFN-g/IL-10 ratio indicated a shift in the Th1/Th2 balance towards a Th2-type response, which might lead to a treatment for Th1-mediated inflammatory immune diseases.Conclusion: Our investigations show that the acetone extract of T. bellerica fruit possesses immunomodulatory activity, which could be used to explain its folklore applications and provide a pharmacological basis for its usefulness in immune-related disorders.Â
Authors and Affiliations
Aurasorn Saraphanchotiwitthaya, Kornkanok Ingkaninan
Keywords
Related Articles
- EP ID EP579305
- DOI -
- Views 84
- Downloads 0
POPULATION STRUCTURE OF KAEMPFERIA GALANGA L. FROM EASTERN INDIA
Objective: India has been a producer of a large number of aromatic medicinal plants which serves as a valuable genetic resource for future quality improvement to meet the ever-growing demand of human essential products....
DEVELOPMENT OF SUSTAINED RELEASE NANOCAPSULES OF CATECHIN RICH EXTRACT FOR ENHANCED BIOAVAILABILITY
Objective: The aim of the study was to develop sustained release nanocapsules of catechin rich extract (CRE) using Eudragit L 100 as a polymer by emulsion solvent evaporation technique with a major focus on enhancing its...
ASSESSMENT OF PATIENT’S KNOWLEDGE REGARDING DISPENSED MEDICATION IN A SOUTH INDIAN GOVERNMENT HOSPITAL
Objectives: Patient’s knowledge regarding medication dispensed to them is an important factor for the proper use of medicines and their well-being, improving the patient’s knowledge, suggesting them with rational ide...
TASTE MASKING BY HOT MELT EXTRUSION WITHOUT LOSS OF BIOAVAILABILITY FOR PEDIATRICS
Objective: The aim of present work was to develop a platform technology for the pediatric dosage form to mask the bitter taste of Furosemide (FUR) and prepare a flexible solid oral dosage form. Methods: Excipient compat...
SIMULTANEOUS DETERMINATION OF NAPROXEN SODIUM AND ACETAMINOPHEN IN FIXED-DOSE COMBINATIONS FORMULATIONS BY FIRST-ORDER DERIVATIVE SPECTROSCOPY: APPLICATION TO DISSOLUTION STUDIES
Objective: To validate and apply a new and easy zero-crossing derivative method for the simultaneous determination of naproxen sodium and acetaminophen in fixed-dose combinations formulations.Methods: Measurement was ach...