Improved Spectrophotometric Pharmacopoeial Method for accurate Quantitation of Thiomersal content in Haemophilus influenzae type b (Hib)-TT Conjugate Vaccine
Journal Title: International Journal of Biomedical and Advance Research - Year 2016, Vol 7, Issue 12
Abstract
Vaccines remain one of the most effective ways to prevent infectious diseases globally. Accurate measurement of thiomersal content is an important parameter to ensure safety of vaccines though these vaccines are less in use. In the present study a simple, accurate and highly effective spectrophotometric improved method has been developed for determination of thiomersal in Hib-TT conjugate vaccine by using 0.01% and 0.005% concentration of dithizone for making stock and comparing these with 0.001% dithizone concentration mentioned in Indian Pharmacopoeia (2014). All the concentrations were evaluated at three different wavelength i.e. 538nm, 540 nm and 542nm. When all the three concentrations compared, the dithizone concentration 0.01 % has been found to be more accurate, linear and giving reproducible results. Among the three wavelengths, the best result has been obtained at wavelength 538nm. The improved method is accurate, specific, and easy to carry out, consumes little time and requires a few microliters of sample and therefore, improved method is recommended than the present pharmacopoeial method which underestimate the concentration of thiomersal and fails the otherwise passed Hib-TT conjugate vaccine.
Authors and Affiliations
Neeraj Malik, Harit Kasana, Veenu Choudhary, Harsh Choudhary, Divya Srivastava, Vandana Tandasi, Anu Prakash, G. R Soni, Surinder Singh
Keywords
Related Articles
- EP ID EP382915
- DOI 10.7439/ijbar.v7i12.3689
- Views 86
- Downloads 0
A correlative study of peptic ulcer and thrombocytopenia in Remo land of Ogun State, Nigeria
Introduction: Helicobacter pylori infection is one of the commonest bacterial infections in human world-wide. H.pylori is a significant cause of a number of intra-digestive and extra-digestive disorders with increased pr...
A Clinical Study of Non Venereal Genital Dermatoses of Adult in a Tertiary Care Center
Aim and Objectives: To study the clinical pattern, aectiological factors, age/sex wise distribution, and the percentage of various non-venereal genital dermatoses and to assess which dermatoses have a predilection for ex...
Intestinal parasitic infections in HIV-1 sero-positive individuals and its correlation with CD4 cell count in a tertiary care teaching hospital, Gujarat
Background: Opportunistic infections constitute a major health problem in patients infected with HIV. Among these, intestinal parasitic infections are the commonest and are a major cause of morbidity and mortality in HIV...
Comparison of clinical performance of I-Gel with LMA-Proseal in elective surgeries under general anesthesia
Aim: To compare insertion characteristics of two different supraglottic devices [I-gel and LMA-Proseal] and to observe any associated complications. Materials and Methods: This prospective, randomized study was conducted...
Study of visual morbidity among the school going children in rural part of Central India
Background: Visual morbidity is an important factor affecting the learning ability, personality and confidence of growing children. Early detection and treatment of visual morbidity is an easy way to improve child health...