Improvements Glutation Levels in the Hippocampus of Aged and Oxidative- Stressed Rats by Supplementation of Alanine-Glutamine Dipeptide
Journal Title: SAINS MEDIKA (Journal of Medicine and Health) - Year 2012, Vol 4, Issue 1
Abstract
Background: Physiological aging or aging due to oxidative stress causes to disruption of glutathione homeostasis of the hippocampus. Disruption of glutathione homeostasis characterized by a decrease in hippocampus glutathione levels below the normal threshold. Decreased levels of glutathione in the hippocampus was demonstrated decreased hippocampus function. One way to increase glutathione levels in the hippocampus and the function of hippocampus is to increase the concentrations of glutathione precursor. One of the glutathione precursors is alanine-glutamine dipeptide. This research was designed to obtain the most effective level of alanine-glutamine dipeptide supplementation to improve glutathione synthesis in the hippocampus of aged and oxidative-stressed rats. The highest glutathione concentrations in the hippocampus showed the best improvement on the hippocampus function in physiological aging or oxidative-stress aging rats. Design and Methods: The experimental rats were assigned into a completely randomized design with 2x2x4 factorial arrangement. The first factor was the age of the experimental rats, consisted of two levels i.e., 12 and 24 months. The second factor was oxidative stress consisted of two levels, i.e., without or with oxidative stress. The third factor was the level of alanine-glutamine dipeptide administration consisted of 4 concentrations, i.e. 0%, 3%, 5%, and 7%. Results: The results showed that administration of 7% alanine-glutamine dipeptide has resulted the highest levels of glutathione hippocampus in younger (0.0154 mg/mg tissue) or aged (0.0140 mg/mg tissue) rats or in normal (0.0150 mg/mg tissue) and in oxidative-stressed (0.0144 mg/mg tissue) rats. The increased hippocampus glutathione levels were associated to the improved functions of the hippocampus. Conclusion: alanine-glutamine dipeptide administration of 7% consentrations gave the best results on repair function of the hippocampus and has the potential to slow aging, both physiological aging or oxidative-stress aging rats (Sains Medika, 4(1):1-12).
Authors and Affiliations
Sunarno . , Wasmen Manalu, Nastiti Kusumorini, Dewi Ratih Agungpriyono
Keywords
Related Articles
- EP ID EP234332
- DOI -
- Views 92
- Downloads 0
The Level of SGOT and SGPT after The Administration of Powdered Colorant Pigment Isolated from Kesumba Keling’s (Bixa Orellana) Seed Coats An experimental study in Balb/C Mice
Background: There has been an effort to explore pigment from kesumba keling’s (Bixa orellana) seed coats for a source of natural food colorant. Consumption of pigment from kesumba keling (Bixa orellana) was assumed to ca...
Calorie Restriction, Stem Cells, and Rejuvenation Approach
-
The Difference in Effectiveness of 70% and 0.5% Chlorine to ReduceThe Germ Number on Stethoscope’s Membrane Experimental research at stethoscope in Baitul Izah Ward of Sultan Agung Islamic Hospital Semarang
Background: Recently, nosocomial infection (hospital- acquired infections) is estimated to affects more than 1.4 million of in patients in all over the world. Stethoscope is one source of nosocomial infection caused by t...
The Effect of Propolis Extract on MDA Levels (Malondialdehyde) and Sperm Quality on Epididimis (Experimental Study on Wistar Strain Male Rats Exposed to Kretek Cigarettes)
Introduction: Cigarette smoke exposure increase free radicals and effecting male infertility. Propolis is commonly used as supplement to increase antioxidant levels. Objectives: To prove the effect of propolis extract to...
Pattern and Frequency of Skin Malignancies at Laboratory of Pathology Anatomy Dr. Kariadi General Hospital in 2008-2009
Introduction: A periodical database is important including for skin cancer. Periodical registration is needed to acknowledge changes in pattern and frequencies of skin lesion. Objective: The purpose of this study was to...