In Silico Expression Analysis of the QTL Region Associated With Root Traits Imparting Drought Tolerance in Rice
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2019, Vol 9, Issue 5
Abstract
Rice (Oryza sativa L.) is the only cereal produced for solely human consumption and is also a cereal of high social and economic value. Water deficit is the main constraint that affects the yield in rice production. Rain fed rice have different drought types and their characterization for the region concerned is essential for identifying drought resistant traits. Therefore, rice varieties with characteristic of efficient use of water are needed to be developed. To understand gene discovery, genetic engineering of rice and mechanism of drought tolerance, fundamental knowledge of plant responses to a biotic stresses at the genomic level is essential. BLAST search was used to obtain the physical position of each flanking QTL interval marker in RGP (Rice Genome Project). All the BAC/PAC clones underlying QTL marker interval were procured and then the genes on sequences were obtained. The expression analysis of the genes of the identified BAC/PAC sequences were performed using MPSS technique. A total of 2568 genes were identified in the putative region of QTLs under study. Genes identified in the putative region of chromosome 1 were 224, chromosome 2 were 388, chromosome 3 were 19, chromosome 4 were 866, chromosome 9 were 1071. Out of these genes, the genes classified as Kinase-like(1), Receptor kinase (1), Transcription factor(8), Aquaporin(1), Protein kinase(11) were chosen for further analysis as it is reported that they play a significant role in drought tolerance.
Authors and Affiliations
Ramole. S, Kotasthane. A, Chouhan. U
Keywords
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- EP ID EP670853
- DOI 10.24247/ijasroct20191
- Views 112
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