In Silico Modeling and Immunoinformatics Probing Disclose the Epitope Based PeptideVaccine Against Zika Virus Envelope Glycoprotein
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2014, Vol 2, Issue 4
Abstract
Zika virus (ZIKV) is an aedes mosquito borne pathogen belonging to the member of flaviviridae subgroup is the causative agent of an emerging disease called Zika fever, known as a benign infection usually presenting as influenza like illness with cutaneous rash. Due to recent epidemic outbreaks it is realized as a major health risk which need enhanced surveillance, but no attempt has been made to design an epitope based peptide vaccine against Zika virus. Viral envelope proteins are derived from host cell membrane proteins with some viral glycoproteins and are used to cover their protective protein capsid, help the viruses to enter host cells and help them to avoid the host immune response. In this study, amino acid sequence of ZIKV envelope glycoprotein was obtained from a protein database and examined with in silico approaches to determine the most immunogenic epitopes for B cell and T cell which could induce humoral as well as cell mediated immune response. Both the linear and conformational epitopes for B cell were predicted by immunoinformatics tools housed in IEDB resources. The peptide sequence DAHAKRQTVVVLGSQEGAV from position 121 and peptide sequence from 117-137 amino acids were predicted as most potential B cell linear and conformational epitopes respectively. Epitopes for CD4+ and CD8+ T cell were also predicted by using tools within IEDB resource and peptide sequence MMLELDPPF from position 250-258 amino acids was predicted as most immunogenic CD8+ T cell epitope with immune response evoking ability prediction score (I pMHC) of 0.09139 and conservancy of 52.17%. The innate immune response for ZIKV envelope glycoprotein was determined by interferon (IFN)-gamma effectuation and mimicking capacity by immunoinformatics and molecular docking study respectively. However, this is an introductory approach to design an epitope based peptide vaccine against Zika virus; we hope this model will be very much helpful in designing and predicting novel vaccine candidate.
Authors and Affiliations
Mohammad Mahfuz Ali Khan Shawan| Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh, Hafij Al Mahmud| Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh, Md. Mahmudul Hasan| Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh, Afroza Parvin| Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh, Md. Nazibur Rahman| Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh, S. M. Badier Rahman| Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
Keywords
Related Articles
- EP ID EP14271
- DOI -
- Views 355
- Downloads 21
Antimicrobial potential of the marine intertidal sponge, Ircinia fusca from west coast of India
In vitro antimicrobial screening of marine sponge (Porifera) Ircinia fusca collected from west coast of India, against selected bacteria and fungi was conducted in this study. Crude sponge extracts of the marine organi...
In-vitro antimicrobial susceptibility testing of bacteria causing diarrhea in children at Khartoum State- Sudan
Background: Diarrhea remains a major public health problem in developing countries. The current study was design to assess the antimicrobial susceptibility testing of bacteria causing diarrhea in children those antibio...
Morphometric Approach towards Growth Performance of Mahseer (Tor tor) in River Narmada Near Hoshangabad (M.P)
During present study total 58 specimens of Mahseer (Tor tor) from River Narmada were studied for morphometric and meristic characteristics. Interrelationship among the morphometric and meristic characteristics were obs...
A review on recent innovation in osmotically controlled drug delivery system
In recent years, oral controlled release (CR) system is most acceptable dosage form by the patients. Drugs having short biological half-life and poor water solubility are the suitable candidate for development of CR sy...
Implementation of time release technology in formulation development and evaluation of sustained release tablet of Lornoxicam
In present study, the attempts have been made to formulate sustained release tablets of lornoxicam by direct compression method. Based on viscosity grades different proportions of hydrophilic polymers (HPMC K4M, HPMC K...