IN SILICO PREDICTION AND VALIDATION OF MICRORNAS FROM JAPANESE ENCEPHALITIS VIRUS (JEV)
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: MicroRNAs are endogenous, small, single stranded, non coding RNAs having 19-25 nucleotides. These miRNAs are complementary to their target messenger RNAs that bind principally to its 3' un translated regions (3’ UTRs). Small RNAs play crucial roles in the regulation of gene expression in many eukaryotes; therefore it is important to predict potential viral miRNAs which might be involved in an establishment of Japanese Encephalitis virus (JEV) disease. Different computational approaches and methods were used for predicting viral microRNAs from the JEV genome in this work.Methods: In the present study, the use of genome-wide computational approach has been demonstrated to predict miRNAs and their target(s) in JEV genome. Two freely accessible softwares, MiPred and Genscan were used to predict the secondary structures of the potential miRNAs.Results: In all, 36 miRNAs were predicted and characterized by conducting genome-wide homology search against all the reported miRNAs. These miRNAs were further validated by performing phylogenetic analyses and using statistical tools.Further, attempt was made to predict the 3′ untranslated regions of mRNAs from whole genome of JEV which may prove helpful in finding putative targets of these miRNAs.Conclusion: This is the first study to identify and validate miRNAs in JEV which is an important step in identifying putative JEV miRNAs that utilize host cell machinery, and may play a crucial role in neuroinflammation and silencing of host genes, thus demonstrating the role of viral miRNAs in establishing viral pathogenesis.Â
Authors and Affiliations
Neema Tufchi, Kumud Pant, Bhasker Pant, Syed Mohsin Waheed
Keywords
Related Articles
- EP ID EP578714
- DOI -
- Views 84
- Downloads 0
INFLUENCE OF BINDING SOLUTION CONCENTRATION, DRYING DURATION AND DRYING TEMPERATURE ON PHYSIOCHEMICAL PERFORMANCE OF NORFLOXACIN GRANULES AND TABLETS
Objective: To investigate the possible individual and joined influences that binding solution concentration, drying temperature and drying duration might have on the physiochemical attributes of granules and tablets usin...
ORAL BIOAVAILABILITY ENHANCEMEMENT OF BROMOCRYPTINE MESYLATE BY SELF-MICRO EMULSIFYING DRUG DELIVERY SYSTEM (SMEDDS)
Objective: The purpose of this work was to enhance oral bioavailability of Bromocryptine Mesylate by preparing SMEDDS (self-micro emulsifying drug delivery system)Methods: Screening of oils, surfactants and co-surfactant...
IN VITRO AND IN SILICO EVALUATION OF THE ANTIDIABETIC EFFECT OF HYDROALCOHOLIC LEAF EXTRACT OF CENTELLA ASIATICA
Objective: Aim of the present study was to explore the possible mechanisms through which Centella asiatica may be beneficial in managing diabetes and its associated complications by in vitro methods and to predict the po...
ANTIMICROBIAL EFFECTS OF INDONESIAN MEDICINAL PLANTS EXTRACTS ON PLANKTONIC AND BIOFILM GROWTH OF PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS
Objective: The increasing rates of antibiotic-resistant microbial infections requires continuous development of new antimicrobial agents. Moreover, microbial biofilms exhibit elevated resistance to most antimicrobial dru...
ENHANCEMENT IN THE COW FEED VALUE OF SOME AGRO-INDUSTRIAL BY-PRODUCTS MEDIATED BY SOLID STATE FERMENTATION WITHTRICHODERMA VIRIDE
Objective: The idea behind this work is to investigate the possible improvement in nutritional values of agro industrial by products namely black gram husk (Vignamungo), yellow pigeon pea husk (Cajanuscajan) and black ch...