IN-VITRO ANTI OXIDANT ACTIVITY OF CHROMATOGRAPHICALLY SEPARATED FRACTIONS FROM SYZYGIUM CUMINI L. LEAVES
Journal Title: Mintage journal of pharmaceutical and medical sciences - Year 2018, Vol 7, Issue 6
Abstract
Aim: Anti-oxidant activity of leaves of Syzygium cumini Linn. (S. cumini L.), a tropical fruit tree of great economic importance possessing several pharmacological properties, is known in literature. We have also found in vitro anti-oxidant activity of methanol extract of S. cumini L. leaves. An attempt was, therefore, made to isolate the anti-oxidant compound from the leaves of M. spicata L. Material & Methods: Leaves of S. cumini L. were processed for isolation of anti-oxidant compound. Solvent extraction, acid hydrolysis, solvent treatment followed by chromatographic experiment were done. Separated fractions in chromatographic experiments were assayed for in vitro anti-oxidant activity by superoxide anion generation with the help of xanthine-xanthine assay, linoleic acid peroxidation assay and DPPH photometric assay. Total phenol, flavonoids, ascorbic acid and carotenoid contents in the separated fractions were also determined. Results: Six fractions were separated in the chromatographic experiment. Fraction number four showed highest anti-oxidant activity which had relation with presence of high amounts of total phenol, flavonoid, ascorbic acid and carotenoids in the fraction. Conclusion: The fraction may be processed further for isolation of anti-oxidant compound.
Authors and Affiliations
Prasenjit Mitra, Prasanta Kumar Mitra, Tanaya Ghosh
Keywords
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