IN VITRO ANTIBACTERIAL ACTIVITY OF CAMELLIA SINENSIS AND ANDROGRAPHIS PANICULATA AGAINST VANCOMYCIN RESISTANT ENTEROCOCCI
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2015, Vol 7, Issue 2
Abstract
The herbal extracts of Camellia sinensis (Cs) and Andrographis paniculata (Ap) with standard F/P ratio (flavonoid/ polyphenol) of 0.26-0.27 were used for evaluation of anti-microbial efficacy against Vancomycin Resistant Enterococci (VRE). The bioactivity fingerprints useful for standardizing both extracts were found to be coherent with respect to their Diphenyl picryl hydrazyl radical scavenging potential (IC50 = ~ 30µg/ml). In vitro efficacy analysis of Cs and Ap extracts using exponentially growing culture of VRE revealed Minimum Inhibitory Concentration (MIC) as 200µg/mL and 50µg/mL respectively. The comparative analysis of their Minimum Bactericidal Concentration (MBC) revealed that 5X concentration of Cs as against 10X concentration of Ap was required to achieve zero microbial count. Such analysis indicated that both extracts would be useful for future analysis. In vivo pre-clinical evaluation studies are underway for utilization of identified herbal leads for trials on humans.
Authors and Affiliations
Pallavi Thakur, Raman Chawla, Ankita Singh Chakotiya, Raghav Nagpal, Ankit Tanwar, Rajeev Goel, Alka Narula, Rajesh Arora, Rakesh K. Sharma
Keywords
Related Articles
- EP ID EP625061
- DOI -
- Views 75
- Downloads 0
METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF NEBIVOLOL HYDROCHLORIDE AND AMLODIPINE BESYLATE IN TABLET DOSAGE FORMS BY RP-HPLC
A simple, specific and precise high performance liquid chromatographic method was developed and validated for Nebivolol HCl and Amlodipine besylate in a combined tablet dosage form. Nebivolol hydrochloride, a long acting...
RENAL VASCULARISATION CAUSING END - STAGE FAILURES AND OUTCOMES: A POOLED ANALYSIS OF COMMUNITY BASED STUDIES
Although acute renal failure (ARF) is believed to be common in the setting of critical illness and is associated with a high risk of death, little is known about its epidemiology and outcome or how these vary in differen...
DEVELOPMENT AND EVALUATION OF MULTILAYERED LAYERED MATRIX TABLETS OF WATER SOLUBLE DRUG
A new approach to zero- order drug delivery that includes geometric factors is described. Systems such as multilayered tablets and other geometrically altered devices have been created to perform this function. The multi...
COMPARATIVE EVALUATION OF POLYHERBAL COMBINATION FOR HYPOLIPIDEMIC ACTIVITY
Hyperlipidemia is defined as increase in the lipid content (group of fat or fat like substances along with their lipoprotein counterpart) in blood. The present study was undertaken to explore the antihyperlipidemic effec...
OPTIMIZATION AND VALIDATION OF MODULATED RELEASE FORMULATION OF RANITIDINE HCL BY RESPONSE SURFACE METHODOLOGY
The objective of the present study was, 1) to systematically device a model of factors that would yield an optimized sustained release dosage form of model drug (Ranitidine HCl), 2) to validate the models using R2 v...