In vitro Anticancer Activity of Hibiscus vitifolius Flowers Ethyl Acetate Fraction against Hepg2 Cell Line
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 9, Issue 5
Abstract
Objective: The roots of Hibiscus vitifolius (Malavaceae) are used for the treatment of jaundice in the folklore system of medicine in India. This study is an attempt to evaluate anticancer activity of the flowers of Hibiscus vitifolius against Liver Cancer cell Line (HepG2). Materials and Methods: In vitro cytotoxicity activity was carried out to screen cytotoxicity potency of the ethyl acetate fraction from Hibiscus vitifolius flower extract at different concentrations against HepG2 cell line. The MTT (methylthiazolyl diphenyl- tetrazolium bromide) assay for cell viability and markers is predictable to confirm the cytotoxicity. Results: The ethyl acetate fraction from the flower extract of Hibiscus vitifolius was tested for its anticancer activity against HepG2 cell lines (liver cancer) at various concentrations by MTT assay. It was confirmed that the IC50 value of this sample was 150 µg/ml against Liver Cancer HepG2 cell line. Conclusion: These findings suggest that the ethyl acetate fraction obtained from the flower EtOH extracts of Hibiscus vitifolius have the potent anticancer activity and thereby justifying its ethnopharmacological claim.
Authors and Affiliations
D. Prabhakaran, A. Rajeshkanna, M. M. Senthamilselvi
Keywords
Related Articles
- EP ID EP342277
- DOI 10.9734/BJPR/2016/20360
- Views 111
- Downloads 0
Phytochemical and Nutritional Composition Analysis of Murraya koenigii Linn Leaves
Vegetable is an indispensable constituent of human diet that supplies the body with minerals, vitamins and certain hormone precursors, in addition to protein and energy. The phytochemical and proximate analyses of Murra...
Antibacterial Potential of Bryophyllum pinnatum Leaf Extracts on Bacteria Obtained from Infected Infant Respiratory Tract
Background: Annual incidence of respiratory infection and death among infants aged between one (1) to two (2) years is increasing, especially in remote areas of the world. Herbal medicines have been the basis of treatmen...
Molecular Typing of Brucella melitensis Isolated from Patients and Animals by Pulsed Field Gel Electrophoresis from Iran
Background: The Brucellosis is recognized as a significant public health problem, with major economic and financial burdens in countries where the disease remains endemic. The disease is one of the most important worldwi...
Alterations in Biochemical Indices and Antioxidant Status in Rats Following Treatment with Gatifloxacin
Aim: Gatifloxacin (GTX) - an 8-methoxy fluoroquinolone antibacterial agent has been considered very effective in the treatment of respiratory and urinary tract infections. This study investigates the toxic potentials of...
Animal Model for Hypoglycemic Studies Using Albino Rats
Introduction: It could be speculated that hypoglycemia increases level of serum α-amylase and α-glucosidase and their corresponding mRNA, with concomitant increase in plasma level of glucagon. However, the concept of dev...