In Vitro Chemical Mutagenesis for Enhancing Variability in Banana Cultivar Nanjanagudu Rasabale
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 5
Abstract
Nanjanagudu Rasabale, also known as Rasthali in Tamil Nadu, is one of the favorite banana cultivar of the Mysore region of Karnataka state. The cultivar belongs to Musa spp., AAB, Silk subgroup, which is under threat of extinction, due its susceptibility to Fusarium wilt Race 1. In order to improve its resistance characters and create variation through mutagenesis, an investigation was carried out at Centre, for Horticulture Biotechnology, Bagalkot. The experiment was conducted with two chemical mutagens (EMS- 0.30%, 0.60%, 0.90% and 1.20% and NaN3- 0.01%, 0.02% and 0.03%), of various concentrations. The lowest plant survival (52.50) and regeneration (87.50 %) was observed, from EMS at 1.20 %. With same EMS concentration, the days taken by the explants for spouting was 5.70 days, whereas, untreated control at 4.20 days. The minimum concentration of NaN3 at 0.01%, recorded the highest (4.70 g) fresh weight and higher doses, invariably have minimum weights. Due to the stimulatory effects, lower concentrations of chemical mutagens, 100 per cent rooting was observed meanwhile, there was no rooting in EMS 1.20 % treated microshoots. The mutagen concentrations of EMS at 0.90 % and NaN3, at 0.01 % have been found to be advantageous in creating variations.
Authors and Affiliations
Kishor. H, Abhijith. Y. C, Manjunatha. N
Keywords
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