In vitro Cytotoxic Activity of Hydro Ethanolic Extract of Delonix regia (Bojer ex. Hook.) Flowers on Cancer Cell Lines
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 4
Abstract
Aims: To evaluate the In vitro cytotoxic effects of the hydro ethanolic extract (HEE) of the Delonix regia flowers against different cell lines. Methodology: The dried Delonix regia flowers are subjected to soxhlet extraction by using 70% ethanol. The dried extract was used to determine the qualitative preliminary phytochemical analysis, total phenolic and flavonoid content. The cytotoxic property of the extract was determined by using MTT assay against breast cancer (MCF-7), cervix (HeLa), brain and colon cancer cells. Tamoxifen is used as a standard for all the cell lines. Results: Qualitative phytochemical tests of extract HEE showed the presence of sugars, flavonoid, tannins, phenolic compounds, steroids and saponins. The percentage of phenolic and flavonoid content was determined as 31.42 mg/g, 29.22 mg/g respectively. The cytotoxic activity of the extract showed, IC50 concentrations (μg/ml) against MCF-7 (breast), carcinoma of cervix HeLa cells, carcinoma of the brain, and carcinoma of colon cells against tamoxifen are 141.6 ± 0.08, 223.7 ± 2.16, 173.9 ± 0.7, 168.33 ± 0.04 respectively. Conclusions: The experimental data clearly demonstrate the hydro ethanolic extract (HEE) showed cytotoxic properties against human cancer cells.
Authors and Affiliations
Madan R. Pusapati, Tejaswi Jonnalagadda, Phani K. Kola, Ankamma C. Yarlagadda, Girijasankar Guntuku
Keywords
Related Articles
- EP ID EP344713
- DOI 10.9734/BJPR/2014/5746
- Views 75
- Downloads 0
Haematinic Potential of Jussiaea repens L – A Search for Antianaemic Herb
Background: Anaemia is a common nutritional disorder. Different harmful synthetic drugs are used to combat it. So herbal remediation is in demand. Aims: In this study a common medicinal herb Jussiaea repens (JR) was used...
Economic Burden of Drug Therapy in Hypertension Management in a Private Teaching Hospital in Nigeria
Background: Information on economic burden of hypertension is needed for relevant decisions and policies due to escalating cost of disease management. Aims: The study assessed economic burden of pharmacotherapy in hype...
Development and Validation of Densitometry TLC Stability Indicating Method for Quantitative Determination of Azelastine Hydrochloride and Emedastine Difumarate in Their Drug Products
Aims: Stability indicating densitometry-TLC assay was established and validated for determination of azelastine hydrochloride (AZT) and emedastine difumarate (ETD) in the presence of their acid and oxidative degradants....
Factors Influencing Job Motivation of Hospital Pharmacists
Aims: The study aimed to explore for factors influencing job motivation of hospital pharmacists. Study Design: A cross sectional descriptive survey. Place and Duration of Study: The study was conducted at Obafemi Awolo...
Phytochemical Testing and In vitro Antibacterial Activity of Bixa orellana (Annatto) Seed Extract
Aim: The present study was designed to investigate the antibacterial activity of the seeds of Bixa orellana (Annatto, family Bixaceae) extracts. Methods: Powdered seed material was extracted using either organic solvents...