In vitro differentiation of human umbilical vein mesenchymal <br /> stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green<br />
Journal Title: Khoon - Year 2010, Vol 7, Issue 1
Abstract
In vitro differentiation of human umbilical vein mesenchymal stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green Raufi A.1, Amini A.1, Azadbakht M.1, Aboozari M.2, Fathi F. 3 1The Faculty of Sciences, Razi University, Kermanshah, Iran 2Kurdistan Digestive Research Center, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran 3Kurdistan Center for Cellular and Molecular Research, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran Abstract Background and Objectives Umbilical cord is one of the major sources of mesenchymal stem cells. In this study, the differentiative potential of human umbilical vein mesenchymal stem cells into hepatocyte-like cells has been investigated by cellular uptake of indocyanine green. Materials and Methods Human umbilical vein mesenchymal stem cells (UVMSCs) were incubated for 2 weeks in the medium containing hepatocyte growth factor; they were also treated with the medium containing oncostatin M for another 2 weeks. The differentiated cells were analyzed by uptake of indocyanine green (ICG), immunofluorescence analysis, and periodic acid-Schiff (PAS) staining. Results The differentiating cells showed transition from bipolar fibroblast-like morphology to round or oval shape. Cellular uptake of ICG was detected in differentiated cells. Glycogen storage was determined by PAS staining in differentiated cells which were immunoreactive to albumin. Conclusions Based on these observations, we can conclude that UVMSCs are able to differentiate into hepatocyte-like cells in vitro and ICG-staining is a useful marker to identify differentiated hepatocytes from human umbilical vein mesenchymal stem cells. Keywords: Cell Differentiation, Umbilical Veins, Mesenchymal Stem Cells, Hepatocytes, Indocyanine Green Sci J Iran Blood Transfus Org 2010; 7(1): 34-40 Received: 21 Jul 2009 Accepted: 27 Feb 2010 Correspondence: Fathi F., PhD of Anatomy. Associate Professor of Cellular and Molecular Research Center, Kurdistan University of Medical Sciences. P.O.Box: 66177-13446, Sanandaj, Iran. Tel: (+98871) 6661830; Fax : (+98871) 6660051 E-mail: farfath@yahoo.com
Authors and Affiliations
A Raufi, M Azadbakht , F Fathi
Keywords
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