In vitro establishment of Bambusa vulgaris var. vulgaris Schrad. ex Wendl. at different times of the year
Journal Title: Biotecnología Vegetal - Year 2010, Vol 10, Issue 3
Abstract
Traditional propagation of Bambusa vulgaris var. vulgaris Schrad. ex Wendl is affected by low percentages of rooting and limited availability of propagules. These elements are handicaps for mass propagation of bamboos. Tissue culture offers a fast and reliable way to develop propagation protocols via organogenesis directly from axillary buds. The objective of this work was to determine the influence of the season to select the initial explant for the in vitro establishment. Two establishments were carried out each month during a whole year (24 at all). The number of buds sprouted and the number of explants free of visible microbial contaminants were quantified after 20 days of culture. Results showed that the season influenced on the in vitro establishment of B. vulgaris var. vulgaris. The highest numbers of buds sprouted (99%) and explants free visible of microbial contaminants (98%) was achieved between January to April and November-December.
Authors and Affiliations
Y. García-Ramírez , M. Freire-Seijo, Blanca Rosa Pérez, Ortelio Hurtado
Keywords
Related Articles
- EP ID EP683812
- DOI -
- Views 117
- Downloads 0
Potential of Bacillus pumilus Bp4185 and Bacillus thuringiensis Bt 3971 for coffee berry borer biocontrol
Coffee berry borer (Hypothenemus hampei), is one of the main pests affecting coffee production worldwide. This pest feeds and reproduces inside the berry reducing its weight and quality, causing losses up to 50%. Some st...
Effect of explant density and volume of culture medium on the in vitro propagation of the hybrid cultivar 'FHIA-21' (AAAB) in Temporary Immersion Systems
The need of producing high quality planting material has required the searching of new alternatives to increase the efficiency of in vitro propagation methods and their automation, such as Temporary Immersion Systems. T...
Conceptualization of technological innovation at the Institute of Plant Biotechnology
An investigation was carried out with the objective of conceptualizing the technological innovation system at Instituto de Biotecnología de las Plantas (IBP), Cuba. A qualitative research methodology was used, which in...
Plant regeneration of five cultivars of Phaseolus vulgaris L. via direct organogenesis
The beans are an important source for human consumption in Cuba due their protein content. For this reason; the search for cultivars with greater potential is a prominent challenge in this specie. Tissue culture in Phas...
Evaluation of different potato somaclones for resistance to Alternaria solani Sorauer, through the use of artificial inoculation under field conditions
The use of plant pathogens, for the genetic improving programs is an important matter to guarantee its success. Utilization of phytopathogenic isolates from natural source, let us to employ rapid screening in some impor...