In vitro establishment of nodal segments of Annona muricata L. young plants
Journal Title: Biotecnología Vegetal - Year 2012, Vol 12, Issue 4
Abstract
The use of tissue in vitro culture for plant propagation of soursop (Annona muricata L.) promissory trees can help increasing the availability of plants for developing field plantations. Considering these aspect, this work aimed to establish in vitro nodal segments of young plants of soursop. Nodal segments with 1.5 cm of length were superficially disinfected with 70% ethanol during one minute and with sodium hypochlorite (0.5, 1.0, 1.5 and 2.0%) during 15 minutes. The presence of microbial contamination and the number of segments with axillary buds were evaluated after 15 and 25 days of culture. The length (cm) of buds was also determined. Results showed a low incidence of microbial contamination in the explants because the presence of fungi in treatments was not observed. However, 3.8% segments were contaminated with bacteria in the treatment with lower concentration of sodium hypochlorite. Axillary shoots were observed in 73.0% of explants when 1.0% of sodium hypochlorite was used, without significant differences using 1.5%. Shoots development with first expanded leaves and a length ranged between 0.8 and 1.5 cm was observed after 25 days of culture. Increasing culture time, plants showed leaf abscission. These results demonstrate that in vitro culture can be used for the propagation of soursop. However, we must make emphasis in the study of the culture conditions for the multiplication phase.
Authors and Affiliations
Leyanis García-Águila , José Manuel Álvarez 2, Yelenys Alvarado-Capó , Milagros González , Mariana La O, Deivis Mirabal , Carlos Romero
Keywords
Related Articles
- EP ID EP683726
- DOI -
- Views 128
- Downloads 0
Identification and control of Bacillus sp., Contaminant of in vitro establishment of Guadua angustifolia Kunth
Efficient protocols of Guadua angustifolia Kunth micropropagation are not reported due to two principal problems for establishing it: explants necrosis and microbial contamination. In this work a high frequent bacterial...
In vitro response of banana plants cv. ‘Grande naine’ (Musa AAA) to water stress induced with polyethylene glycol
Bananas and plantains are grown in tropical and subtropical regions of the world. These plants required high humid conditions; therefore water deficit affects their growth and productivity. Through biotechnology, new d...
Mycobiota of donor plants and fungal contaminants from the in vitro establishment of bamboo species
The presence of fungal contaminants may limit the establishment of bamboos explants. Knowing the contaminant microorganisms present in donor plants allow to trace more appropriate control strategies. This work was aimed...
Influence of the isolate on the phytotoxicity of the filtrate of the culture of Alternaria solani Sor. on potato plants var. ‘Desirée’
Growth in a PDA medium of six isolate of A. solani, coming from the microbial cultivation collection of the IBP, and the phytotoxic of culture filtrate of each one were studied to evaluate the influence of isolate in the...
Evaluation in two production areas of potato somaclones obtained by mutagesis and somaclonal variation
With the objective to evaluate, in field, the behaviuor face to Early blight and the agronomics and morphologic characteristics of two somaclones, the IBP-27, obtained by mutagenesis in vitro, and the IBP-10 by somaclona...