In Vitro Evaluation of Cytotoxic and Antiproliferative Effects of <i>Portulaca oleracea</i> Ethanolic Extracton on HeLa Cell Line
Journal Title: Gene, Cell and Tissue - Year 2017, Vol 4, Issue 1
Abstract
Background Purslane (<i>Portulaca oleracea</i>) is an ubiquitous garden weed that has been traditionally used as an antidiabetic and anti-inflammation agent. However, its potential antiproliferative and cytotoxic effects against cancerous cells are still unclear. Objectives This study aimed to evaluate the antitumoral activity of the ethanolic extract of aerial parts of <i>P. oleracea </i>on HeLa cell line. Methods In this study, HeLa cells were cultured and exposed to various concentrations of <i>P. oleracea </i>ethanolic extract for 24 and 48 hours. Cytotoxicity and cell viability were evaluated, using MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and trypan blue, respectively. Results <i>P. oleracea </i>ethanolic extract effectively decreased proliferation of HeLa cell (P < 0.001). It also decreased the viability of the cells after treatment with the extract (P < 0.001). Ethanolic extract of <i>P. oleracea </i>exerts its time and dose dependent effects. Conclusions It is suggested that <i>P. oleracea </i>has antiproliferative effects and could affect cell cycle of HeLa cell line. However, some issues about its optimal dose and time, its efficiency compared to routine cancer therapy medicines and its behavior against normal cells should be clarified before its clinical application as a therapeutic agent.
Authors and Affiliations
Shiva Khatibi, Zahra Farzaneh Taban, Amaneh Mohammadi Roushandeh
Keywords
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