In vitro Evaluations of Cytotoxicity and Anti-inflammatory Effects of Peganum harmala Seed Extracts in THP-1-derived Macrophages
Journal Title: European Journal of Medicinal Plants - Year 2015, Vol 5, Issue 2
Abstract
Aim: Based on knowledge from traditional Greco-Arab herbal medicine, this in vitro study aims to evaluate the cytotoxicity and the anti-inflammatory mechanism of Peganum harmala (P. harmala) seeds by measuring the expression and release of both anti-inflammatory (IL-10) and pro-inflammatory (IL-1, IL-2, IL-6 and TNF-) cytokines in human THP-1-derived macrophages. Methodology: Cells were treated with 1g lipopolyssacharide/ml (LPS) in the absence and presence of increasing concentrations of extracts from the P. harmala seeds (PHS-extract). During the entire experimental period, we used extract concentrations (up to 250 g/ml) that had no cytotoxic effects as measured with MTT and LDH assays. The anti-inflammatory effects were assessed by measuring the levels of secretory proteins and mRNA of IL-1, IL-2, IL-6, IL-10 and TNF-in LPS-activated (1g/ml) human THP-1-derived macrophages. Results: PHS-extract remarkably increased IL-10 release and mRNA expression at a concentration of 64g/ml and suppressed the expression and release of IL-1, IL-6 and TNF- in dose-dependent manner. No significant effects on the expression levels of IL-2 were observed after treatment with PHS-extract. Conclusions: These results indicate that PHS-extract probably exert anti-inflammatory properties throughout increasing the release and expression of IL-10 mRNA as well as by suppressing IL-1, IL-6 and TNF- mRNA expression in THP-1 cells.
Authors and Affiliations
Shahinaz Mahajna, Mohammad Azab, Hilal Zaid, Basheer Abo Farich, Feras Al Battah, Shiri Mashner, Bashar Saad
Keywords
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- EP ID EP350291
- DOI 10.9734/EJMP/2015/13267
- Views 121
- Downloads 0
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