Abstract

Objective: Antioxidant capacities and phenolic contents of Gallic Acid isolated from plant Caesalpinia decapetala were investigated. Caesalpinia decapetala is a plant used to treat burns, biliousness and stomach disorders. Methods: The antioxidant activity was estimated by the ABTS and DPPH methods. The total phenolic was measured using Folin-Ciocalteu assay. Identification of isolated Gallic Acid was also performed by reverse-phase high-performance liquid chromatography (RP-HPLC) using Cosmosil C18 column (150mm x 4.6mm, 5毺 m particle). The mobile phase was a mixture of ethyl acetate: ethanol: water (1:5:4, v/v/v) delivered at a flow rate of 1.0 mL min-1. Results: The total phenolic content was found to be 4.31% (w/w). Isolation of Gallic Acid with optimum yield was performed using a mixture of solvents (Ethanol: Water 65:35). Isolated Gallic Acid showed significant in vitro free radical scavenging activity in both model; but in ABTS assay significant % inhibition of free radical was observed as compared to DPPH assay. Conclusions: Research concluded that Caesalpinia decapetala extract can be used as potent antioxidant which can play vital role against the diseases like neurodegenerative disorders, inflammation, viral infections and gastric ulcer.

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