In vitro Investigation of Antimicrobial, Antitumor and DPPH Reduction Capacity of the Methanolic Extract of Scoparia dulcis
Journal Title: Microbiology Research Journal International - Year 2015, Vol 10, Issue 3
Abstract
Scoparia dulcis belongs to the family Plantaginaceae. It is a flowering plant and locally common in the treatment of gastrointestinal upset and malaria. The aim of this study was to investigate the antimicrobial, antitumor and antioxidant activity of methanolic leaf extract of S. dulcis. The DPPH free radical scavenging assay was used to evaluate antioxidant property. The disk diffusion method and brine shrimp lethality bioassay were used to determine antibacterial and cytotoxic activity, respectively. In the evaluation of antioxidant property IC50 was found to be 196.66 µg/ml, in cytotoxicity testing, it was found that the plant extract has 29.868 µg/ml of LC50. The methanolic extract of S. dulcis leaves also have efficiency in bacterial growth inhibition; this extract is effective against both gram negative and positive bacteria. The zones of inhibition at 500 µg/ml dose in E. coli and S. aureus culture were 20 mm and 22 mm, respectively. In thin layer chromatography analysis, presence of non-polar and polar compounds, three non-chromatophoric compounds were found. We conclude that the S. dulcis is a potent antibacterial, antioxidant and cytotoxic medicinal plant.
Authors and Affiliations
A. K. M. Riasat-ul-Islam, Prawej Ansari, Awale Yusuf Mousa, S. M. Nazmul Haque, Nasrin Sultana, Md. Nasir Uddin, Mahmuda Sultana
Keywords
Related Articles
- EP ID EP354774
- DOI 10.9734/BMRJ/2015/19437
- Views 69
- Downloads 0
Microorganisms Isolated during Fermentation of Sorghum for Production of Akamu (A Nigerian Fermented Gruel)
Aim: The aim of this study is to isolate microorganisms present during fermentation of sorghum for production of akamu. Place and Duration: The study was done at Nsukka, Enugu State for a period of ten weeks. Methodology...
Biochemical and Molecular Characterization of Food Contaminating Bacteria Isolates from Food Stall Vegetables
Today the issue of food safety is global problem that gets main concern in setting public health policy. The eruption of diseases caused by food contamination occurs in places where sanitation and hygiene conditions are...
Marine Environment: A Potential Pool of Isolating Antibiotic Producing Bacteria with Novel Characteristics
Aims: This review article analyze the data based on antibiotic producing bacteria isolated from different poles of marine environment globally and further more their antibiotic potential against different pathogenic micr...
Detection of Carbapenem Resistance in Salmonella Species from a Tertiary Hospital in Eastern Cape, South Africa
Aims: Broad-spectrum carbapenem group is the current therapy for strains of Enterobacteriaceae that express extended spectrum beta-lactamases (ESBLs). However, recent reports of therapeutic failures of carbapenems with s...
The p53 Codon 72 Polymorphism in Moroccan Women and the Risk of Ovarian Cancer
The aim of this study was to examine the association of the p53 codon 72 polymorphism with ovarian carcinoma development in Moroccan women. Samples from 44 women with ovarian cancer and 80 healthy controls, were used. p5...