In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line

Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2020, Vol 17, Issue 5

Abstract

Objectives: There are several types of pesticides to control pests and several new types coming into use that could be less toxic compared to the old ones. Pesticide-induced oxidative stress, which is one of the main mechanisms of toxicity, is the research area focused most on over the last decade. There are several different studies in the literature on whether pesticide exposure induces oxidative stress parameter-mediated toxicity. Pesticide-induced oxidative stress level depends on the biochemical features of mammalian systems. Imidacloprid is a neonicotinoid pesticide in wide use that is considered safe; however, it has been reported in different studies that it may cause changes in oxidative stress parameters. Materials and Methods: We investigated the dose- and time-dependent effects of imidacloprid on acetylcholinesterase (AChE), lactate dehydrogenase (LDH), and glutathione (GSH) levels in the L-929 fibroblast cell line. The effects of 1-500 μg imidacloprid dose range on AChE, GSH, and LDH were investigated. Results: LDH levels were significantly increased dose dependently in the 250 and 500 ng imidacloprid groups compared to the control group. GSH levels nonsignificantly decreased dose dependently and GSH levels were lower in the 500 ng imidacloprid group compared to the control group. There were no significant differences between the groups in AChE levels. Conclusion: These results indicated that high doses of imidacloprid may induce oxidative stress in fibroblast cells.

Authors and Affiliations

Çiğdem SEVİM, Ali TAGHİZADEHGHALEHJOUGHİ, Mehtap KARA

Keywords

Related Articles

Some New Hydrazone Derivatives Bearing the 1,2,4-Triazole Moiety as Potential Antimycobacterial Agents

Objectives: The aim of this study was to synthesize, characterize, and screen some new 1-(4-((2-(4-substitutedphenyl)hydrazono)methyl)phenyl)-1H-1,2,4-triazole derivatives for their antimycobacterial activities. Materia...

A Controlled Release Theophylline Delivery System Based on a Bilayer Floating System

Objectives: Bilayer floating drug delivery is an approach that helps to overcome the shortcomings of single-layered tablets. There is little or no fluctuation of the drug in the blood stream or tissue, while control is e...

A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach for Estimation of Phytochemicals in Extracts of Moringa Oleifera Leaves

Objectives: A systematic design of experiment (DoE) based sensitive, robust high performance thin layer chromatographic (HPTLC) method was established for simultaneous estimation of gallic acid (GA), quercetin (QT), and...

The Effect of Rusa unicolor Antler Deer Extracts from East Kalimantan in Bone Turnover Cell Models

Objectives: Osteoporosis is a condition characterized by skeletal degradation of osseous tissue resulting in an escalated chance of broken bones. Traditionally, Rusa unicolor horn from East Kalimantan is used to treat ma...

Investigation of the Antioxidant, α-Glucosidase Inhibitory, Anti-inflammatory, and DNA Protective Properties of Vaccinium arctostaphylos L.

Objectives: The scope of this study was to investigate the total phenolic, anthocyanin, and flavonoid contents and the biological properties of ethanol extract (EE), methanol extract (ME), and aqueous extract (AE) from V...

Download PDF file
  • EP ID EP689992
  • DOI 10.4274/tjps.galenos.2019.15807
  • Views 259
  • Downloads 0

How To Cite

Çiğdem SEVİM, Ali TAGHİZADEHGHALEHJOUGHİ, Mehtap KARA (2020). In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. Turkish Journal of Pharmaceutical Sciences, 17(5), -. https://europub.co.uk./articles/-A-689992