In vitro propagation of bastard balm (Melittis melissophyllum L.)
Journal Title: Herba Polonica - Year 2015, Vol 61, Issue 3
Abstract
An efficient method for in vitro propagation of bastard balm by enhanced axillary shoot branching has been developed. The material to establish in vitro culture was shoot tips collected from three-year-old plants in May. The shoots obtained from initial explants were placed on MS/B5 medium containing 0.1, 0.5 or 1.0 mg/l BA with 0.01 mg/l NAA or without the auxin. The highest number of shoots per explant was obtained on the medium with 1.0 mg/l BA (3.9 shoots per explant). For the rooting of shoots ½ MS/B5 with IBA (0.25, 0.50 and 1.0 mg/l) medium was used. The medium without plant growth regulators served as a control. The best root regeneration was observed on the medium without IBA (87.1% of cuttings rooted). IBA used in the medium for shoot rooting affected the morphological traits of obtained microcuttings but not affected their weight. Irrespective of auxin concentration in this medium, obtained microcuttings acclimated in ex vitro conditions very well.
Authors and Affiliations
Anna Pawełczak, Izabela Szymborska-Sandhu, Katarzyna Bączek, Zenon Węglarz
Keywords
Related Articles
- EP ID EP266772
- DOI 10.1515/hepo-2015-0023
- Views 70
- Downloads 0
9α-hydroxyparthenolide in Zoegea leptaurea subsp. mesopotamica (Czerep.) Rech. (Asteraceae)
From the aerial parts of Zoegea leptaurea subsp. mesopotamica (Czerep.) Rech. (syn. Zoegea mesopotamica Czerep.), 9α-hydroxyparthenolide was isolated. This compound was identified by spectral methods (1H NMR and 13C NMR)...
Law regulations concerning food supplements, dietetic food and novel food containing herbal substances
Food supplements are concentrated sources of nutrients and/or other substances with a nutritional or physiological effect. However, they often contain herbal substances or their preparations. Food supplements belong to c...
In vitro antimicrobial activity of extracts and their fractions from three Eryngium L. species
Introduction: Due to increasing resistance against antibiotics and antifungal agents, crude plant extracts, fractions, and isolated pure compounds became a new interest as antimicrobial agents. Objectives: The antimicrob...
Occurrence of pathogenic and endophytic fungi and their influence on quality of medicinal plants applied in management of neurological diseases and mental disorders
Due to increasing demand of medicinal plants (MPs), quality and safety more attention to the plant health should be paid. Among herb pathogens, especially fungi cause serious diseases in these plants decreasing yield and...
Comparison of chemical compositions of essential oils isolated by hydrodistillation from wild thyme (Thymus serpyllum L.) with use of Deryng and Clevenger apparatus
The aim of the studies conducted in 2012-2013 was to compare the chemical composition of essential oils isolated from wild thyme (Thymus serpyllum L.) by hydrodistillation in Deryng and Clevenger apparatus. GC-MS analysi...