In vitro propagation of the Cuban variety of rice Jucarito-104
Journal Title: Biotecnología Vegetal - Year 2007, Vol 7, Issue 2
Abstract
In vitro propagation is an efficient method of propagating disease-free, genetically uniform and massive amounts of in vitro plants, irrespective of the year season. This study was carried to develop a micropropagation system for the cuban Jucarito-104 rice cultivar and to evaluate the response of rice in vitro plantlets to different cytokinins (6- BAP and kinetin) and liquid or semisolid MS culture media, in order to identify treatments to achieve shoot multiplication. Best results were obtained using 5.0mg.l -1 of 6-BAP in MS liquid medium: seven axillary buds per initial explants after seven days of in vitro culture of plantlets. Acclimatization was acceptable without adding auxins on rooting media in order to obtain 100% of plants with normal root. This result may facilitate the application of biotechnological approaches for improving this rice cultivar.
Authors and Affiliations
Maylin Pérez Bernal , Yamilet Coll García , Annerys González Quintero , Carlos Hernández Díaz , Raúl Armas Ramos , Merardo Pujol Ferrer
Keywords
Related Articles
- EP ID EP684089
- DOI -
- Views 141
- Downloads 0
Determination of the minimum lethal doses of phosphineotricine for selection of banana transformants cv. ‘Grande naine’ (Musa AAA)
The present work had the objective of determining the minimum lethal doses of the selective agent phosphineotricine, to establish the adequate conditions for the selection of genetically transformed cellular embryogenic...
In vitro radiosensitivity of two types of common bean explants of ‘BAT- 93’ cultivar
The use of Gamma radiations combined with in vitro tissue culture is a useful tool in breeding programs. However, for its application it is necessary to have an efficient plant regeneration system. The objective of thi...
Protocol for isolation of Moniliophthora roreri (Cif and Par) Evans et al. from cacao fruits cv. ‘National’ in the Ecuadorian Amazonia
Moniliophthora roreri is a pathogen of cocoa (Theobroma cacao L.) that causes high economic losses in Ecuador. This paper is intended to present a protocol for the isolation of the pathogen from cocoa fruits cv. ‘Natio...
Optimization of the culture medium and fermentation conditions for Pseudomonas fluorescens-based biofertilizers production
In Cuba, a biofertilizer has been developed based on a Pseudomonas fluorescens strain phosphate solubilizer. Given the need to standardize its industrial scale up, this work aimed to optimize the culture medium and the f...
Diversity of cultivable microorganisms isolated from soil in bamboo plantations
Bamboos have a great economic importance due to their applications and their impact on the environment, mainly in protecting soil and watersheds. However, microbial communities associated with plantations of bamboo speci...